This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from Cytiva and how to analyze the refolding process with Superdex columns from Cytiva.
This page shows how to purify NAD+-dependent dehydrogenases and ATP-dependent kinases by affinity chromatography using 5’ AMP Sepharose® 4B, HiTrap® Blue HP and Blue Sepharose® 6 Fast Flow products from Cytiva.
Sera-Mag SpeedBeads Protein A/G Magnetic Particles provide a fast and convenient method for both manual and automated magnetic isolation of proteins using affinity binding. The particles can be used for isolating antibodies from serum, cell culture supernatant or ascites, and
This page shows how to perform a separation with a sephacryl column from Cytiva which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.
Monoclonal antibodies (MAb) continue to gain importance as therapeutic and diagnostic agents for many cancers. The process of screening hybridoma libraries for candidate MAbs typically requires several days to complete and can be labor-intensive when evaluating multiple MAbs in parallel.
To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin