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Manual and Automated Purification
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
Peptide Impurities
Peptide Impurities
Centrifugation-free Protein Extraction and Purification from Total Cultures of Baculovirus-infected Insect Cells
Centrifugation-free Protein Extraction and Purification from Total Cultures of Baculovirus-infected Insect Cells
The Potential of Dendritic Polyester Scaffolds as Biocompatible Drug Delivery Agents
Professor Michael Malkoch provides an overview of the unique properties of dendritic molecules and their application in biomedical fields. He highlights bis-MPA dendritic scaffolds as a promising biodegradable and biocompatible platform for drug delivery applications
RPC Purification Options and Scale up with SOURCE™ Media
The different stages of working with SOURCE™ (Cytiva) for high-resolution separation are discussed, from purification to polishing and scaling up
Anti-FLAG® M2 Magnetic Beads
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
General Considerations for Purification of GST-tagged Protein
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
Salt Selection and Buffer Preparation
The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority
Purification of Thiol-Containing Substances By Covalent Chromatography
Thiol-containing substances can be isolated selectively by covalent binding to an activated thiolated matrix via thiol-disulphide exchange to form a mixed disulphide bond. After washing away unbound material, the thiol-containing substance is eluted by reducing the disulphide bond. This technique
Principles and Standard Conditions for Different Purification Techniques
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins
Troubleshooting of methods for purification of MBP-tagged recombinant proteins using Cytiva products.
Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON® Columns
His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.
Troubleshooting Purification Methods
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
Deglycosylation Kits
Deglycosylation Kits
Protein Labeling and Purification with Amicon® Pro Centrifugal Filters
Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.
Protein G and Protein A Bind to Different IgG
This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.
Ion Exchange Chromatography Troubleshooting
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Extract-N-Amp™ Product Guide
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Principles and Standard Conditions for Different Purification Techniques
This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from Cytiva.
Affinity Chromatography Troubleshooting
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Purification of Membrane Proteins
This page shows how to perform a purification of His-tagged membrane proteins.
IgG Antibody Purification with HiTrap® Protein G HP Columns
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
Troubleshooting Detection Methods for GST-tagged Proteins
This page describes troubleshooting strategies in the detection of GST-tagged proteins.
Performing an Isolation of Recombinant Protein Complexes
This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.
Troubleshooting Guide for Affinity Chromatography of Tagged Proteins
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
Purification or Removal of Serine Proteases, such as Thrombin and Trypsin, and Zymogens
This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from Cytiva.
Performing a Separation with IgG Sepharose 6 Fast Flow
Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification
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