Protein quantitation

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Counterstaining after the Duolink In Situ Protocol

We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

LC-UV-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC-Mimic

SigmaMab Antibody Drug Conjugate Mimic, is a non-toxic drug mimic utilized as a standard for mass spectrometry and high performance liquid chromatography.

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.

Duolink® PLA Probemaker Guide

Guide to create custom PLA probes for immunofluorescent or brightfield detection.

Protein Determination by the Bicinchoninic Acid (BCA) Method

A standard procedure for the quantitative determination of total protein concentration of a solution by the BCA method.

Duolink® PLA Multicolor Detection Protocol

Using Duolink® in Protein Interaction Studies

The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.

Protein Determination using 2-D Quant Kit

2-D Quant Kit from Cytiva is designed to accurately determine protein concentrations in samples for electrophoresis.

Creating PLA® Probes

Duolink® kits use in situ PLA® technology for accurate quantification of individual proteins, interactions, and modifications in cells and tissue.

Production of Dual-labelled Proteins

Refinement of protein dual-labeling protocol using EnPresso® B Defined Nitrogen-free showcased by Vernalis, Ltd. for drug discovery.

Duolink® PLA Brightfield Protocol

Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.