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Molecularly Imprinted Polymer SPE- Acid NSAIDs from Muscle
A new, successfully developed and validated LC-MS-MS method allowing the extraction of NSAIDs from poultry muscle tissue using Molecularly Imprinted Polymer (MIP) SPE.
A Comparison of Solid Phase Extraction (SPE) Products Used to Purify Peanut Paste Extract for Aflatoxin Analysis
Supel Tox AflaZea SPE produced exceptional analyte recoveries with good reproducibility for all aflatoxins while requiring less than half the sample volume required by competing products.HPLC analysis of all spiked samples after SPE cleanup showed that all compared SPE cartridges...
Chiral and Achiral LC-MS Analysis of Warfarin™ in Plasma Samples
Clinical laboratories are beginning to reap the benefits of LC-MS in terms of its sensitivity, specificity, throughput, and potential to reduce cost per sample. The purpose of this study was to demonstrate the LC-MS analysis of Warfarin in plasma samples...
Reference Standards for Analyzing Polyphenol Catechins
Reference Standards for Analyzing Polyphenol Catechins
The Extraction of Amphetamine and Related Drugs using Molecularly Imprinted Polymer SPE
The Extraction of Amphetamine and Related Drugs using Molecularly Imprinted Polymer SPE
Determination of Neonicotinoids in Honey using a Chromolith RP-18 HPLC column and UV Detection
Determination of Neonicotinoids in Honey using a Chromolith RP-18 HPLC column and UV Detection
UHPLC Analysis of Conjugated Estrogens on Ascentis ® Express C18
Separation of Estriol 3-(β-D-glucuronide) sodium salt; β-Estradiol 3-(β-D-glucuronide) 17-sulfate dipotassium salt; Estriol 3-sulfate sodium salt; β-Estradiol 3,17-disulfate dipotassium salt, ≥95%; β-Estradiol 17-(β-D-glucuronide) sodium salt; β-Estradiol 3-(β-D-glucuronide) sodium salt; Estrone 3-(β-D-glucuronide) sodium salt; β-Estradiol 3-sulfate sodium salt, ≥93%; Estriol, ≥97%; Estrone...
Rapid Determination of Protein Binding Affinity Using Solid Phase Microextraction
Accurate protein binding affinities were measured using bioSPME more rapidly than membrane dialysis (RED) techniques. Binding affinities correlated to the reference values.
HybridSPE-Phospholipid Technology
This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.
Class-Selective Enantiomeric Separation of beta-Blockers and beta-Agonists using CHIROBIOTIC T Stationary Phase
Understanding the role of chirality in pharmacology became extremely important following the tragedy of thalidomide use in the 1960s. At that time the effect of chirality was poorly understood, and whilst it was discovered that the sedative effect resided in...
Ascentis ® Express HILIC U/HPLC Columns
For separation of polar compounds including polar neutrals, polar acids, and polar and non-polar basic amines use our Ascentis® Express HILIC column.
LC/MS/MS Method for Determination of Glyphosate, AMPA, and Glufosinate in Cereals
Glyphosate and related compounds are measured in oatmeal and infant cereal using ion-exchange polymer-based particles for HPLC and SPE. Low level detection was obtained.
Supel-Select HLB SPE for Pharmaceutical Analysis
Select HLB SPE is a hydrophilic polymer phase for analyte extraction from aqueous matrices. Here it’s used for statins in plasma and 48 pharmaceutical analytes in drinking water.
Bioanalysis with SPME
Biocompatible SPME devices employ C18 bonded porous silica sorbent particles, in a binder which resists fouling by biological matrix components
Quick, Sensitive LC/MS/MS Analysis of Aflatoxins in Cannabis
Analyte recovery values from spiked cannabis extracts ranged from 102 to 127% with RSD below 12% for 3 replicates. Excellent recovery values were even observed for Aflatoxins B2 and G2 which were spiked at a low level of 6.1 ppb.
Investigating Matrix Interference in Analysis of Antiarrhythmic Cardiac Drugs in Plasma
For efficient therapeutic drug monitoring, it is important for clinicians to have access to fast and robust analytical methods for accurate assessment of drug efficacy. Industrial trends toward highly specific LC/MS applications over traditional immunoassay have resulted in the need...
Two Distinct Sample Prep Approaches to Overcome Matrix Effect in LC/MS of Serum or Plasma Samples
This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.
HPLC Analysis of Sibutramine Enantiomers on Astec® CHIROBIOTIC® V
Separation of Sibutramine hydrochloride monohydrate, ≥98% (HPLC), solid
Evaluation of LCMSMS Deuterium Scrambling in Clinically Significant Small Molecules
LC-MS/MS is a powerful tool that brings numerous benefits to the clinical sample analysis arena. However, due to the complexity of the instrumentation there are some unique challenges that also accompany these benefits. Even following sample extraction and cleanup, matrix...
LC/MS/MS Analysis of Testosterone and Metabolites on Ascentis® Express C18
Separation of Testosterone solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material; 17α-Methyltestosterone solution, 1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material; 5α-Dihydrotestosterone (DHT) solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified...
LC/MS Analysis of Ethanol Metabolites in Urine on Ascentis® Express OH5 after Dilution with Deuterated Internal Standards
The method shown uses HILIC mode on an Ascentis® Express OH5 column to retain both analytes well, making it very likely to be more robust and reliable, as well as highly MS-friendly.
HPLC Analysis of Cisplatin and Monohydrated Cisplatin on SeQuant ZIC-HILIC
Antineoplastic agents separated with excellent resolution.
LC/MS Analysis of Antiarrhythmic Drugs and Metabolites on Ascentis® Express HILIC
The benefit of HILIC over traditional reversed-phase chromatography is two-fold for both sample introduction and analyte detection. First, the high acetonitrile concentration of HILIC mobile phases allows for direct analysis of precipitated plasma samples without the need for additional sample...
HPLC Analysis of Xanthines on Purospher® STAR RP-18 endcapped
Xanthine is a purine base found in most human body tissues and fluids as well as in other organisms. Methylated xanthines (methylxanthines), which include caffeine, paraxanthine, theobromine, and theophylline, commonly used for their effects as mild stiµlants and as bronchodilators...
HPLC Analysis of Z-Drugs on Ascentis® Express ES-Cyano 2.0 μm
An rapid method for the siµLtaneous determination of the Z-drugs or sleep aids: zopiclone, zolpidem, and zaleplon is presented here. The need for greater analytical capacity and throughput for the analysis of sleep aid medicines (Z-drugs) in forensic toxicology laboratories...
Chiral Separation of the Pesticide Diniconazole by Supercritical Fluid Chromatography (SFC)
Diniconazole is a pesticide commonly used as a fungicide to prevent various plant diseases. It is a chiral compound that exists as a pair of enantiomers. This article describes the use of ChromegaChiral™ columns in resolving the two diniconazole enantiomers...
Detection, Measurement and Regulation of NOGE (Novolac Glycidyl Ethers) and Other Packaging-Derived Epoxy Compounds In Food Products
Detection, Measurement and Regulation of NOGE (Novolac Glycidyl Ethers) and Other Packaging-Derived Epoxy Compounds In Food Products
Benefits of Ascentis Express Phenyl-Hexyl over Biphenyl for the Separation of Pain Management Opiates
Although both biphenyl and phenyl-hexyl phases can resolve these compounds, the former exhibits excellent peak shape and substantially less silanol-derived ion exchange activity.
UHPLC Analysis of Cannabinoids on Ascentis Express C18
As the popularity of cannabis-infused products increases, there is a growing need to characterize the type and content of the cannabinoids found in the product. This application demonstrates the ability of the Ascentis Express C18 column to baseline resolve...
Ascentis® Express RP-Amide Columns for HPLC and UHPLC
Improve your separation of basic compounds with our Ascentis® Express RP-Amide HPLC columns from the Supelco® portfolio.
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