Protocol for using Casein Blocking Buffer in Southern and Western blotting applications, including colorimetric and chemiluminescent substrate detection options. Troubleshooting for optimizing signal and reducing background is also included.
Measure and compare the activity of proteases with this non-specific protease activity assay using casein. Use this assay as a standardized procedure to determine the activity of proteases for quality control.
Western blot protocol with workflow steps for different blot procedures, describing the electrophoretic transfer of proteins from SDS polyacrylamide gels to sheets of nitrocellulose. Also known as Immunoblotting protcol.
5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) is an ideal insoluble substrate for use with alkaline phosphatase. This system produces a blue-purple product. The intense color can be observed visually, is very stable, and will not fade upon exposure to light.