qPCR

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RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.

Primer and Probe Design

Video: Primer and Probe Design

Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

KiCqStart^® Probe Assays Frequently Asked Questions

Custom Oligos Modifications

We offer over 200 custom DNA oligos modification for both DNA, RNA oligonucleotides and sequencing to probes for gene.

KAPA SYBR® FAST qPCR Kits FAQs

Review some of the most asked questions regarding the KAPA SYBR FAST qPCR kits, which provide optimal performance under strict real-time PCR reaction conditions, and improve the signal-to-noise ratio (fluorescence), quantitation cycle (Cq), linearity and sensitivity.

RT-PCR Troubleshooting

PCR troubleshooting guide for reliable data in molecular assays.

How qPCR Works

Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.

PCR/qPCR/dPCR Assay Design

PCR workflow's variability influenced by sample source, reverse transcription, and assay design, impacting reproducibility.

MIQE: Real-Time PCR Experiment Standards

MIQE-compliant qPCR services support primer design, protocol development, and data analysis for faster product development and publication.

Array CGH Analysis of Challenging Samples

aCGH technology enhances chromosomal analysis, requiring significant DNA input per sample.

Probe-based qPCR

Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.

DNA Oligonucleotide Synthesis

Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis.

PCR/qPCR Data Analysis

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

Oligonucleotide Quality Control by Mass Spectrometry

Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.

Choosing the Right Custom qPCR Probe

View a summary of the applications using commonly ordered custom qPCR probes.

Universal ProbeLibrary System Technology

UPL is based on only 165 short hydrolysis probes. They are labeled at the 5' end with fluorescein (FAM) and at the 3' end with a dark quencher dye.

PCR Assay Optimization and Validation

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Troubleshooting PCR and RT-PCR Amplification

This page shows PCR and RT-PCR amplification troubleshooting.

Complete Solutions for PCR Assay Development

Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.

Direct and Crude Extract qPCR

Rapid qPCR analysis from highly inhibited tissue and blood sample extractions with KAPA PROBE.

Kapa Biosystems qPCR Reagents and Kits

Quantitative PCR (qPCR) and qRT-PCR reagents and kits that are designed to deliver maximum efficiency and reliable results from a variety of genetic materials.

RNA Immunoprecipitation (RIP) FAQs

RNA Immunoprecipitation (RIP) Frequently Asked Questions

PCR Technologies Guide

Basic PCR/qPCR/dPCR protocols for assay quality control and specific studies.

Molecular Beacons

Learn about precision Sigma-Aldrich Molecular Beacon oligonucleotide probes used for sequence detection in qPCR for in vitro research.

KiCqStart^® SYBR^® Green Primers FAQs

Primer pairs detect prevalent eukaryotic genes, but may co-amplify other transcripts, emphasizing the importance of comprehensive primer design.

Dual-Labeled Probes

Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.

Probe QPCR for Gene-Level Expression

qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.

Locked Nucleic Acid & Minor Groove Binder / Eclipse Dark Quencher

Explore Locked Nucleic Acid and MGB. These oligo modifications offer stability and specificity in molecular analysis, making them ideal for research and diagnostic applications.

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