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EN 14105: GC Analysis of Glycerin Impurity in Biodiesel on MET-Biodiesel
EN 14105: GC Analysis of Glycerin Impurity in Biodiesel on MET-Biodiesel
High-throughput Real-Time PCR: SYBR® Green
High-throughput qPCR using SYBR® Green is a demanding application that requires consistent and reproducible results from low-volume, high-speed assays.
Purification using StrepTrap™ HP 1 mL and 5 mL
This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from Cytiva.
HPLC Analysis of Various Cationic Polymers on TSKgel® PWXL-CP Columns
Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan
Overcoming Aggregation in Solid-phase Peptide Synthesis
The ease of assembly of a given peptide sequence is hard to predict, which makes peptide synthesis challenging. Review methods and reagents for avoiding aggregation in solid-phase peptide synthesis.
Determination of l- and d-Lactic Acid Enantiomers by HPLC/MS/MS
Rapid, simple, reproducible chiral separation and quantification of L- and D-lactic acid enantiomers using Supelco’s Astec CHIROBIOTIC® R chiral column and tandem MS.
Multiplex Real-Time PCR
Multiplex qPCR employing probe-based chemistries is a demanding application that often requires extensive optimization and validation.
Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
SYBR® Green JumpStart™ Taq ReadyMix™
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
Rapid Genotyping of Mouse Tissue Using Extract-N-Amp Tissue PCR Kit
Genomic detection of DNA via PCR amplification and detection on an electrophoretic gel is a standard way that the genotype of a tissue sample is determined. Conventional preparation of tissues for PCR-ready DNA often take several hours to days, depending
Negative Photoresist Procedure
Our photoresist kit was designed to have the necessary chemical components for each step in the lithographic process. The component materials are provided in pre-weighed quantities for your convenience. Etchants are available separately so that the proper etchant can be
Preparing Cells for Electroporation
Preparing Cells for Electroporation
Nucleic Acid Electrophoresis Protocols & Introduction
An introduction to Nucleric Acid electrophoresis and standard protocols for Agarose gel electrophoresis for DNA, RNA & polyacrylamide gel electrophoresis for DNA.
Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit
The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Urine Concentration with Amicon® Ultra Centrifugal Filters
Accurate measurement of specific proteins in urine is important for diagnosing and managing diseases.
Concepts and Tools for RAFT Polymerization
We present an article about RAFT, or Reversible Addition/Fragmentation Chain Transfer, which is a form of living radical polymerization.
REDExtract-N-Amp™ Tissue PCR Kit Protocol
The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.
DIG RNA Labeling Kit (SP6/T7) Protocol & Troubleshooting
Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).
Imprint® Methylated DNA Quantification Kit (MDQ1) Protocol
Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol
GenElute™ Gel Extraction procedure
The GenElute Gel Extraction kit is designed for the rapid purification of 50 bp to 10 kb linear DNA fragments and plasmids from standard or low-melting agarose gels.
pGEX Vectors
This page describes the characteristics of pGEX expression vectors used with Cytiva products.
Casein Blocking Buffer Protocol for Southern or Western Blotting
Protocol for using Casein Blocking Buffer in Southern and Western blotting applications, including colorimetric and chemiluminescent substrate detection options. Troubleshooting for optimizing signal and reducing background is also included.
Protocol for Purification, Optional Reduction, and SEC-MS Analysis of a Monoclonal Antibody
A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.
Protector RNase Inhibitor Protocol & Troubleshooting
The addition of Protector RNase Inhibitor to cell pellets is not recommended. Protector RNase Inhibitor as a 50kDa protein will most probably not permeate into intact cells. If the cell pellet is immediately frozen at -80 °C, this will be
New EN ISO 22964 for Cronobacter spp. detection in the food chain
The new EN ISO 22964:2017 standard specifies a horizontal method for the detection of Cronobacter spp. in food, animal feed and environmental samples.
SIG10 Chemically Competent Cells
For best results, ligation reactions must be heat inactivated at 70º C for 15 minutes before transformation. Alternately, the reactions may be purified.
Simplicon™ RNA Transfection and Electroporation Protocols
Simplicon™ RNA Transfection and Electroporation Protocols
SYBR® Green Extract-N-Amp™ Tissue PCR Kit Protocol
The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
DNase I Recombinant, RNase-free Protocol
DNase I from bovine pancreas is a glycoprotein of Mr 37000. A special procedure is used to remove RNases from the DNase preparation.
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