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Showing 1-30 of 41 results for "ficoll 400" within Technical Documents
Product Information Sheet - F4375
f4375pis.doc FICOLL 400* Product Number F4375 Storage Temperature RT CAS#: 26873-85-8 Product Description Ficoll 400 is a highly branched polymer formed by the copolymerization of
Product Information Sheet - F4375
Sheet Ficoll® 400 Catalog Number F4375 Product Description Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely
Human PBMC Isolation and Counting Using the Scepter™ 2.0 Handheld Automated Cell Counter
paramount that clean separation of the blood and Ficoll® layers is maintained prior to centrifugation 5. Centrifuge without the brake applied at 400 g x 30 min at 18–24°C. Note: Higher temperatures
Faster Isolation of PBMC Using Ficoll-Paque® Plus in the Eppendorf Multipurpose Benchtop Centrifuges 5920 R and 5910 Ri
centrifugation using Ficoll-Paque PLUS. Ficoll-Paque has a density of 1.007 g/mL. Due to their higher density, erythrocytes, granulocytes and dead cells will pass through the Ficoll layer, whereas lymphocytes
Data Sheet - D7058
blue and xylene cyanol FF. Ready for use in agarose gels. Reagents Storage Buffer: 2.5% ficoll, type 400, containing 0.0125% bromophenol blue, and 0.00625% xylene cyanol FF. Storage/Stability
Data Sheet - D3937
2,500 2,000 1,500 1,000 500 Reagents Storage Buffer: Solution in 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol FF.
Product Information Sheet-collp-ro
HBSS containing 10 mM HEPES and 5% FCS (fetal calf serum). Step 3, 4, 8 Ficoll gradient 25% stock solution (Ficoll 400 in HBSS containing 10 mM HEPES) and 23%, 20%, and 11% dilutions. Steps
Data Sheet - D7808
100 µg/ml) • 1 ml vial of 6X Sample Loading Buffer, Product Code B 6924, containing 15% (w/v) Ficoll, 0.06% (w/v) bromophenol blue, 0.06% (w/v) xylene cyanol FF, 30 mM EDTA. Storage/Stability Markers
Enhanced MALDI-TOF MS Spectra of Serum Peptides Using Ultrafiltration ; ASMS 2002
plasma samples were taken from healthy male and female volunteer donors. Ficoll was added to blood samples and centrifuged at 500xg. Ficoll diluted plasma was separated and keep frozen at -20°C. Trifluoroacetic
Product Information Sheet-11814389001
extractions or chaotropic agents. • Particularly useful for high-volume research currently requiring Ficoll-Hypaque gradients. https://www.sigmaaldrich.com 2. How to Use this Product y Version
Product Information Sheet - PCG3005
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
Product Information Sheet - PCG2010
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
Product Information Sheet - PCG2007
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
PCG3001 - Bulletin
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
Product Information Sheet - PCG2001
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
Product Information Sheet - PCG2003
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
Product Information Sheet - PCG2006
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
Product Information Sheet - PCG2009
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
Product Information Sheet - PCG2017
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
Product Information Sheet - PCG2008
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
Product Information Sheet - PCG2004
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
Product Information Sheet - PCG2005
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
Product Information Sheet - PCG2002
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
Product Information Sheet - PCG2011
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
Product Information Sheet - PCG2019
Catalog Number G5516) 4% (w/v) Lithium Dodecyl Sulfate (LDS) (Catalog Number L9781) 4% Ficoll 400 (Catalog Number F2637) 0.025% Phenol Red (Catalog Number P3532) 0.025% Brilliant Blue G250
Immunomonitoring using the Scepter 2.0 Cell Counter and Software Pro
-buffered saline (1X EmbryoMax® PBS, MilliporeSigma) and layered over 15 mL of Ficoll®. Samples were centrifuged at 400 x g for 30 minutes with no brake, and the resulting PBMC layer was recovered.
History of Western Blotting
™ LDS Sample Buffer 4.0 g glycerol 0.40 g lithium dodecyl sulfate 1.2 g triethanolamine 0.40 g Ficoll® 400 2.5 mg Phenol Red 2.5 mg Brilliant Blue G250 7.0 mg EDTA Milli-Q® water to final volume 10 mL The
Molecular Biology Reagents Insert: OmniPur®
$105 $2001 OmniPur® Dextran Sulfate, 50% Solution Sterile 3730 100 ml $177 OmniPur® Ficoll® 400 4550 4555 50 g 500 g $295 $3187 OmniPur® D-(-)-Ribose 7410 100 g $153 OmniPur® Sucrose
Product Information Sheet-11718096001
saline (PBS*) • Immunohistocytochemistry • Western blotting • In situ hybridization 50x FPG 1% Ficoll 400 1% polyvinylpyrrolidone 360 1% glycine In situ hybridization 20x SSC buffer 150 mM NaCl
ekko system - how it works
wash on the ekko™ System, or manual processing via centrifugation followed by PBMC isolation using Ficoll-Paque (Cytiva®). Growth and viability was found to be equivalent for T cells isolated from acoustically
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