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m3183

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Jingqi Chen et al.
Microbial cell factories, 14, 92-92 (2015-06-27)
Secretory expression of valuable proteins by B. subtilis and its related species has attracted intensive work over the past three decades. Although very high yields can be achieved with homologous proteins, production of heterologous proteins by B. subtilis is unfortunately
Shewit Kalayou et al.
Cell biology and toxicology, 30(6), 361-376 (2014-11-25)
The mycotoxin alternariol (AOH) is an important contaminant of fruits and cereal products. The current study sought to address the effect of a non-toxic AOH concentration on the proteome of the steroidogenic H295R cell model. Quantitative proteomics based on stable
Constantine Chrysostomou et al.
ACS chemical biology, 10(3), 875-882 (2014-12-18)
Microbial arsenate resistance is known to be conferred by specialized oxidoreductase enzymes termed arsenate reductases. We carried out a genetic selection on media supplemented with sodium arsenate for multicopy genes that can confer growth to E. coli mutant cells lacking
Justin P Ludeman et al.
Organic & biomolecular chemistry, 13(7), 2162-2169 (2014-12-24)
Tyrosine sulfation is a widespread post-translational modification that mediates the interactions of secreted and membrane-associated proteins in such varied biological processes as peptide hormone action, adhesion, blood coagulation, complement activation and regulation of leukocyte trafficking. Due to the heterogeneous nature
Md Kamrul H Chowdhury et al.
Archives of physiology and biochemistry, 121(3), 88-96 (2015-07-03)
In the last 20 years the prevalence of metabolic disorders, in particular type 2 diabetes (T2D), has more than doubled. Recently, a strong link between T2D and cancer, in particularly liver cancer has been reported. However, the mechanism connecting the
Katelyn T Gause et al.
ACS nano, 9(3), 2433-2444 (2015-02-26)
The development of subunit antigen delivery formulations has become an important research endeavor, especially in cases where a whole cell vaccine approach has significant biosafety issues. Particle-based systems have shown particular efficacy due to their inherent immunogenicity. In some cases
Katrijn De Brucker et al.
Antimicrobial agents and chemotherapy, 58(9), 5395-5404 (2014-07-02)
We identified a 26-amino-acid truncated form of the 34-amino-acid cathelicidin-related antimicrobial peptide (CRAMP) in the islets of Langerhans of the murine pancreas. This peptide, P318, shares 67% identity with the LL-37 human antimicrobial peptide. As LL-37 displays antimicrobial and antibiofilm
Fang Zhang et al.
PloS one, 10(7), e0132948-e0132948 (2015-07-22)
Bacteria predominantly reside in microbial communities known as biofilms, where cells are encapsulated and protected by the extracellular matrix (ECM). While all biofilm cells benefit from the ECM, only a subgroup of cells carries the burden of producing this public
Claine L Snow et al.
Journal of inorganic biochemistry, 105(7), 972-977 (2011-05-13)
The buffer used during horse spleen ferritin iron loading significantly influences the mineralization process and the quantity of iron deposited in ferritin. Ferritin iron loading in imidazole shows a rapid hyperbolic curve in contrast to iron loading in 3-(N-morpholino)propanesulfonic acid
Go Atsumi et al.
The Journal of general virology, 96(Pt 2), 431-439 (2014-10-30)
In this study, we identified a novel virus from gentian (Gentiana triflora) that causes ring-spots on ovaries. Furthermore, the virus causes unusual symptoms, ring-spots that appear specifically on the outer surface of the ovarian wall after pollination. Pollen grains carrying
David L Jakeman et al.
Journal of industrial microbiology & biotechnology, 33(9), 767-772 (2006-03-29)
The jadomycins are a unique family of benzoxazolophenanthridine antibiotics produced by Streptomyces venezuelae ISP5230 following heat or ethanol shock or phage infection. We have modified the culture conditions by altering the carbon source, buffer, inoculum size, and timing of ethanol
Seyedeh Mahdieh Hashemi et al.
Bioorganic & medicinal chemistry, 23(7), 1481-1491 (2015-03-06)
In order to find new azole antifungals, we have recently designed a series of triazole alcohols in which one of the 1,2,4-triazol-1-yl group in fluconazole structure has been replaced with 4-amino-5-aryl-3-mercapto-1,2,4-triazole motif. In this paper, we focused on the structural
Christopher H Douse et al.
ChemMedChem, 10(1), 134-143 (2014-11-05)
Motility is a vital feature of the complex life cycle of Plasmodium falciparum, the apicomplexan parasite that causes human malaria. Processes such as host cell invasion are thought to be powered by a conserved actomyosin motor (containing myosin A or myoA)
D E Di Croce et al.
Naunyn-Schmiedeberg's archives of pharmacology, 387(9), 873-881 (2014-06-21)
Myotoxic effects related to intracellular Ca(2+) disturbances have been reported for local anesthetics. Such effects might derive from Ca-ATPase dysfunction. The aim of this work was to describe the effect of lidocaine and bupivacaine on the sarcoplasmic reticulum (SR) Ca-ATPase
Chih-Cheng Hsiao et al.
Cancer letters, 368(1), 144-152 (2015-08-16)
Toll-like receptor-4 (TLR4) is known to influence growth and migration of hepatocellular tumors; however, its role in hepatoblastoma remains poorly understood. This study investigated the regulatory role of TLR4 in proliferation and chemoresistance of HepG2 hepatoblastoma cells. Treatment with lipopolysaccharide
Eduardo Padilla-Camberos et al.
BioMed research international, 2015, 837452-837452 (2015-06-17)
Cholesterol control is fundamental for prevention of cardiovascular disorders. In this work, the hypocholesterolemic activity of an aqueous Opuntia ficus-indica extract (AOE) was tested in triton-induced mice. The inhibitory activity on pancreatic lipase enzyme was evaluated in vitro by the
Don L Tucker et al.
Journal of bacteriology, 184(23), 6551-6558 (2002-11-12)
Escherichia coli MG1655 acid-inducible genes were identified by whole-genome expression profiling. Cultures were grown to the mid-logarithmic phase on acidified glucose minimal medium, conditions that induce glutamate-dependent acid resistance (AR), while the other AR systems are either repressed or not
Changgui Li et al.
Human vaccines & immunotherapeutics, 11(6), 1351-1356 (2015-05-15)
The outbreak of human infections of a novel avian influenza virus A (H7N9) prompted the development of the vaccines against this virus. Like all types of influenza vaccines, H7N9 vaccine must be tested for its potency prior to being used
Oddrun Elise Olsen et al.
Cell communication and signaling : CCS, 13, 27-27 (2015-06-07)
Activins are members of the TGF-β family of ligands that have multiple biological functions in embryonic stem cells as well as in differentiated tissue. Serum levels of activin A were found to be elevated in pathological conditions such as cachexia
Helena Kaihola et al.
PloS one, 10(1), e0116459-e0116459 (2015-01-23)
Depressive symptoms during pregnancy are common and may have impact on the developing child. Selective serotonin reuptake inhibitors (SSRIs) are the most prescribed antidepressant treatment, but unfortunately, these treatments can also negatively affect the behavioral development and health of a
Djalila Mekahli et al.
Pflugers Archiv : European journal of physiology, 466(8), 1591-1604 (2013-11-07)
Autosomal dominant polycystic kidney disease (ADPKD) is caused by loss-of-function mutations in either PKD1 or PKD2 genes, which encode polycystin-1 (TRPP1) and polycystin-2 (TRPP2), respectively. Increased activity of the mammalian target of rapamycin (mTOR) pathway has been shown in PKD1
María José Varela Liste et al.
Molecular pharmacology, 87(6), 936-953 (2015-03-15)
G protein-coupled receptors, including the M3 muscarinic acetylcholine receptor, can form homo-oligomers. However, the basis of these interactions and the overall organizational structure of such oligomers are poorly understood. Combinations of site-directed mutagenesis and homogenous time-resolved fluorescence resonance energy transfer
Belén López-García et al.
Applied microbiology and biotechnology, 99(19), 8011-8021 (2015-04-08)
There are short cationic and tryptophan-rich antifungal peptides such as the hexapeptide PAF26 (RKKWFW) that have selective toxicity and cell penetration properties against fungal cells. This study demonstrates that concatemeric peptides with tandem repeats of the heptapeptide PAF54 (which is
Jérémy A Ferreira Barbosa et al.
Virology journal, 12, 188-188 (2015-11-19)
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important pathogens in the swine industry and causes important economic losses. No effective antiviral drugs against it are commercially available. We recently reported that the culture supernatant of
Hui Liu et al.
International journal of medical microbiology : IJMM, 305(3), 392-403 (2015-03-26)
Helicobacter pylori from different individuals exhibits substantial genetic diversity. However, the kinetics of bacterial diversification after infection with a single strain is poorly understood. We investigated evolution of H. pylori following long-term infection in the primate stomach; Rhesus macaques were
Katarzyna Brodowska et al.
International journal of oncology, 45(6), 2311-2324 (2014-09-13)
Recent studies suggest that the anti-diabetic drug metformin may reduce the risk of cancer and have anti-proliferative effects for some but not all cancers. In this study, we examined the effects of metformin on human retinoblastoma cell proliferation in vitro
Stefanie Eggers et al.
European journal of human genetics : EJHG, 23(4), 486-493 (2014-08-08)
Disorders of sex development (DSDs) encompass a broad spectrum of conditions affecting the development of the gonads and genitalia. The underlying causes for DSDs include gain or loss of function variants in genes responsible for gonad development or steroidogenesis. Most
Alyssa K Van Dreumel et al.
Journal of clinical microbiology, 53(6), 1797-1805 (2015-03-20)
An amino acid consensus sequence for the seven serotypes of foot-and-mouth disease virus (FMDV) nonstructural protein 3B, including all three contiguous repeats, and its use in the development of a pan-serotype diagnostic test for all seven FMDV serotypes are described.
João B A Neto et al.
PloS one, 9(5), e93698-e93698 (2014-05-13)
In recent decades, the incidence of candidemia in tertiary hospitals worldwide has substantially increased. These infections are a major cause of morbidity and mortality; in addition, they prolong hospital stays and raise the costs associated with treatment. Studies have reported
Aled E L Roberts et al.
The Journal of antimicrobial chemotherapy, 70(3), 716-725 (2014-11-19)
Manuka honey is a broad-spectrum antimicrobial agent that seems to affect different bacteria in many different ways. It has been shown to be bactericidal against Pseudomonas aeruginosa by destabilizing the cell wall, but we aimed to investigate whether there were
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