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Quantitative PCR Basics
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
Ago RIP to Isolate microRNA and their Targets Using Imprint® RNA Immunoprecipitation Kit
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
PCR Master Mix
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
MystiCq® MicroRNA® Quantitation System
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents