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Lysis and protoplast formation of group B streptococci by mutanolysin.

Infection and immunity (1980-06-01)
G B Calandra, R M Cole
ABSTRACT

Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls, simultaneously produced peculiar effects of cytoplasmic coagulation, retention of cell shape, loss of some intracellular enzymes, and a rise in optical density. Addition of purified mutanolysin to the array of muralysins (group C streptococcal phage-associated lysin, lysozyme), previously successful in preparing protoplasts of different streptococci, now makes possible enzymatic preparation of protoplasts of streptococci of groups A, B, C. D. G, and H.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Mutanolysin from Streptomyces globisporus ATCC 21553, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)
Sigma-Aldrich
Mutanolysin from Streptomyces globisporus ATCC 21553, lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified