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Manipulating Cellular Activities Using an Ultrasound-Chemical Hybrid Tool.

ACS synthetic biology (2017-09-26)
Ching-Hsiang Fan, Yao-Shen Huang, Wei-En Huang, Albert Alexander Lee, Sheng-Yang Ho, Yu-Lin Kao, Cuei-Ling Wang, Yen-Ling Lian, Tasuku Ueno, Tsung-Shing Andrew Wang, Chih-Kuang Yeh, Yu-Chun Lin
ABSTRACT

We developed an ultrasound-chemical hybrid tool to precisely manipulate cellular activities. A focused ultrasound coupled with gas-filled microbubbles was used to rapidly trigger the influx of membrane-impermeable chemical dimerizers into living cells to regulate protein dimerization and location without inducing noticeable toxicity. With this system, we demonstrated the successful modulation of phospholipid metabolism triggered by a short pulse of ultrasound exposure. Our technique offers a powerful and versatile tool for using ultrasound to spatiotemporally manipulate the cellular physiology in living cells.

MATERIALS
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Sigma-Aldrich
1,2-Distearoyl-sn-glycero-3-phosphoethanolamine, ≥99%