Electric currents, wires, leads, combs, leaks… so many opportunities for trouble. But we still use gels, because electrophoresis remains an effective way to separate proteins — so that the results of antibody-based immunodetection can be fairly unequivocal.
Click on the Sample Preparation & Gel Electrophoresis topics to read about the possible causes and remedies:
No Bands or Gel Front
Sample Doesn't Sink to the Bottom of the Well
Sample Leaking Out of Well
Bands are Smeared Vertically
Too Many Bands
Gel Running Unusually Slowly
Gel Running Unusually Fast
Protein Bands Too Close Together (Not Completely Resolved)
Leftmost and/or Rightmost Bands of the Gel are Distorted
Bands Form Smiling Shapes
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