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Authenticated Colorectal Cancer Cell Lines for Cancer Research

Colorectal cancer (CRC) is the second most common cancer in women, and the third most common in men. For statistics combining men and women, CRC is the fourth leading cause of cancer mortality. More than half of the CRC cases reported are in developed countries, with the incidence increasing in developing countries. Colorectal cancer accounts for 10% of cancer-related deaths in western countries. Despite innovative therapeutic advances, mortality rates for colorectal cancer patients remains high1.

Types of cancer

Most colorectal cancers are classified as adenocarcinomas. Other, less common, types include gastrointestinal stromal tumor (GIST), carcinoid or neuroendocrine tumors, small cell carcinomas, and lymphomas, which typically start in cells of the immune system, but can also arise in other organs such as the colon or rectum1.

Cancer cell panels listed below are derived from adenocarcinoma.

Risk factors

Etiology and risk factors are both genetic and environmental. There is an increase in the incidence of colorectal cancer if first-degree family members are positive for the disease1.  Lifestyle factors like smoking, alcohol consumption and obesity also increase the incidence of colorectal cancer.

Mutations

The most common genes altered (mutations with frequency >30%) in colorectal cancer includes APC, TP53, ERBB2, KRAS, PTEN, and BRAF.

Choose cell lines from the table below based on mutation, and click genes to find relevant products (antibodies, shRNA, siRNA, primers, CRISPR plasmids) for your research study.

Mutant geneCell lines
APCCACO2, COLO201, COLO205, GP2D, GP5D, HCT15, HT29, HT55, LOVO, LS123, LS180, MDST8, SW1116, 
SW1417, SW403, SW48, SW480, SW620, SW948, T84
TP53CACO2, HCT15, HT115, HT29, HT55, LS123, SW1116, SW1417, SW403, SW480, SW620, SW948, T84
ERBB2COLO205, LS180, SW480
KRASGP2D, GP5D, HCT116, HCT15, LOVO, LS123, LS180, SW1116, SW403, SW480, SW620, SW948, T84
PTENGP2D, GP5D, LS180, MDST8
BRAFCOLO201, COLO205, GP2D, GP5D, HCT116, HT115, HT29, HT55, LOVO, LS180, MDST8, SW1417
Table1. Colorectal cancer cell lines with specific somatic mutations

Small molecules/monoclonal antibodies

Small molecule compounds and antibodies can be used to target specific cancer cells and block tumor growth and progression. The most common drugs used to target colorectal cancer include:

Applications

Cancer cell lines are the foundation for cancer research, and provide an accessible, cost-effective model for cellular behavior and response. Based on the characteristics of the cell line and experimental need, cell lines may be used in one or more applications. Some examples of application-specific cell line use are included below.

ApplicationCell line used
Drug response studiesSpheroid models developed from Caco-2, DLD-1, COLO 205, HT-29 and HCT116  mimic the tumor environment and were employed to study drug response2
Evaluation of new treatment strategiesLS174 cell line was used as a model of colon carcinoma to evaluate the efficacy of anti-TAG-72 immunoliposomes3
CR4, a highly tumorigenic cell line, provided an important tool for the development of novel therapeutic strategies4
Target identification/validationSW480 cell line was used to investigate  β-catenin as a novel target for cancer therapy5
Targeted drug deliverySW480  was employed to evaluate the efficacy of doxorubicin in conjugation with EGF receptor-binding peptide in doxorubicin-resistant cell lines (targeted delivery)6
Growth factor signalingHT29-MTX-E12 in the undifferentiated state acts as model for cell-nutrient interaction studies, and was used to study the effect of growth factors on intestinal cell viability7
Cell migration studiesThe role of JNK signaling in cell migration and invasion was studied in the  COLO 205 cell line8
In vitro modelsOE-19 cell line was used as model for Barrett’s carcinogenesis to study the role of ghrelin on disease pathogenesis9
Metastasis studiesCOLO 205 cell line was used as model to study the role of E-cadherin in cancer metastasis10
Product No.Cell NameCell Line Origin
86010202CACO-2Human Caucasian colon adenocarcinoma
9042001CACO-2Human Caucasian colon adenocarcinoma, intestinal permeability characteristics tested
87091201COLO 201Human Caucasian colon adenocarcinoma
87061208COLO 205Human Caucasian colon adenocarcinoma
93052620COLO 206FHuman colon carcinoma
93051118COLO 320 DMFHuman colon carcinoma
87061205COLO 320DMHuman Caucasian colon adenocarcinoma
87101501COLO 320HSRHuman Caucasian colon adenocarcinoma
93052621COLO 741Human colon carcinoma
90062901DHD/K12/TRbRat colonic carcinoma
90102540DLD-1Human colon adenocarcinoma
95090714GP2dHuman Caucasian colon adenocarcinoma
95090715GP5dHuman Caucasian colon adenocarcinoma
6061901HCA-2Human sigmoid colon adenocarcinoma
6061903HCA-24Human colon adenocarcinoma
7031601HCA-46Human colon adenocarcinoma
6061902HCA-7Human colon adenocarcinoma
9071501HCA-7 Colony 1Human colorectal adenocarcinoma
9071506HCA-7 Colony 11Human colorectal adenocarcinoma
9071509HCA-7 Colony 24Human colorectal adenocarcinoma
9071511HCA-7 Colony 26Human colorectal adenocarcinoma
9071513HCA-7 Colony 27Human colorectal adenocarcinoma
2091238HCA-7 Colony 29Human colon carcinoma (subpopulation isolated from the HCA-7 cell line)
9071502HCA-7 Colony 3Human colorectal adenocarcinoma
9071516HCA-7 Colony 30Human colorectal adenocarcinoma
9071504HCA-7 Colony 6Human colorectal adenocarcinoma
91091005HCT 116Human colon carcinoma
91030712HCT-15Human colon adenocarcinoma
85061104HT115Human colon carcinoma
91072201HT29Human Caucasian colon adenocarcinoma
92012401HT29 gluc C1Human Caucasian colon adenocarcinoma
85061109HT29/219Human Caucasian colon carcinoma
85061105HT55Human colon carcinoma
88090801IA-Xs SBRHoltzmann rat small bowel adenocarcinoma
10092301LIM1215Human colorectal carcinoma
87060101LoVoHuman colon adenocarcinoma
94120801LS 123Human colon adenocarcinoma
87060401LS174THuman Caucasian colon adenocarcinoma
87021202LS180Human Caucasian colon adenocarcinoma
99011801MDST8Human colon carcinoma
87071006SW 1116Human Caucasian colon adenocarcinoma
90102543SW 1417Human colon adenocarcinoma
87071008SW 403Human Caucasian colon adenocarcinoma
89012702SW 48Human colon adenocarcinoma
87092801SW 480Human colon adenocarcinoma
87051203SW 620Human Caucasian colon adenocarcinoma
91030714SW 948Human Caucasian colon adenocarcinoma
88021101T84Human colon carcinoma
91011802WB2054MRat colon carcinoma
85111501WiDrHuman colon adenocarcinoma

References

1.
Kuipers EJ, Grady WM, Lieberman D, Seufferlein T, Sung JJ, Boelens PG, van de Velde CJH, Watanabe T. 2015. Colorectal cancer. Nat Rev Dis Primers. 1(1): https://doi.org/10.1038/nrdp.2015.65
2.
Hoffmann OI, Ilmberger C, Magosch S, Joka M, Jauch K, Mayer B. 2015. Impact of the spheroid model complexity on drug response. Journal of Biotechnology. 20514-23. https://doi.org/10.1016/j.jbiotec.2015.02.029
3.
Kim KS, Lee YK, Kim JS, Koo KH, Hong HJ, Park YS. 2008. Targeted gene therapy of LS174?T human colon carcinoma by anti-TAG-72 immunoliposomes. Cancer Gene Ther. 15(5):331-340. https://doi.org/10.1038/cgt.2008.11
4.
Rowehl RA, Burke S, Bialkowska AB, Pettet DW, Rowehl L, Li E, Antoniou E, Zhang Y, Bergamaschi R, Shroyer KR, et al. Establishment of Highly Tumorigenic Human Colorectal Cancer Cell Line (CR4) with Properties of Putative Cancer Stem Cells. PLoS ONE. 9(6):e99091. https://doi.org/10.1371/journal.pone.0099091
5.
LI K, ZHOU Z, JI P, LUO H. 2016. Knockdown of ?-catenin by siRNA influences proliferation, apoptosis and invasion of the colon cancer cell line SW480. 11(6):3896-3900. https://doi.org/10.3892/ol.2016.4481
6.
Ai S, Jia T, Ai W, Duan J, Liu Y, Chen J, Liu X, Yang F, Tian Y, Huang Z. 2013. Targeted delivery of doxorubicin through conjugation with EGF receptor-binding peptide overcomes drug resistance in human colon cancer cells. Br J Pharmacol. 168(7):1719-1735. https://doi.org/10.1111/bph.12055
7.
Giromini C, Baldi A, Fusi E, Rebucci R, Purup S. 2015. Effect of growth factors, estradiol 17-?, and short chain fatty acids on the intestinal HT29-MTX cells. Cell Biol Toxicol. 31(4-5):199-209. https://doi.org/10.1007/s10565-015-9304-y
8.
ZHANG Y, LIN L, JIN Y, LIN Y, CAO Y, ZHENG C. 2016. Overexpression of WNT5B promotes COLO 205 cell migration and invasion through the JNK signaling pathway. 36(1):23-30. https://doi.org/10.3892/or.2016.4772
9.
Konturek PC, Burnat G, Rau T, Hahn EG, Konturek S. 2008. Effect of Adiponectin and Ghrelin on Apoptosis of Barrett Adenocarcinoma Cell Line. Dig Dis Sci. 53(3):597-605. https://doi.org/10.1007/s10620-007-9922-1
10.
Petrova YI, Schecterson L, Gumbiner BM. 2016. Roles for E-cadherin cell surface regulation in cancer. MBoC. 27(21):3233-3244. https://doi.org/10.1091/mbc.e16-01-0058
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