Designing of peptide substrates. Different approaches exemplified by new chromogenic substrates for kallikreins and urokinase.

Chromogenic peptide substrates for serine proteases have been designed by using two approaches: (1) by using the natural substrate as a model and (2) by structure-activity correlations obtained through screening of a large number of tripeptides. Some recent examples, substrates for kallikreins and urokinase are given.