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Bei der SPE werden ein oder mehrere Analyten aus einer flüssigen Probe durch Extraktion, Partitionierung und/oder Adsorption an eine feste stationäre Phase isoliert. * * * ## Verwandte Produkte Slide 1 of 22 1 of 6 [ \ Supelco \ 575659-U \ HybridSPE®-PLus 96-Well Plate](https://www.sigmaaldrich.com/CH/de/product/supelco/575659u) Schnellansicht [ \ Supelco \ 57057 \ Supelclean™ LC-Florisil® SPE Tube](https://www.sigmaaldrich.com/CH/de/product/supelco/57057) Schnellansicht [ \ Supelco \ 57226 \ Supelclean™ENVI™-Chrom P SPE Tube](https://www.sigmaaldrich.com/CH/de/product/supelco/57226) Schnellansicht [ \ Supelco \ 54024-U \ Supelclean™ ENVI-Carb/NH2 SPE Tube](https://www.sigmaaldrich.com/CH/de/product/supelco/54024u) Schnellansicht [ \ Supelco \ 54035-U \ Supelclean™ ENVI-Carb/NH2 SPE Tube](https://www.sigmaaldrich.com/CH/de/product/supelco/54035u) Schnellansicht [ \ Supelco \ 57088 \ Supelclean™ ENVI-Carb™ SPE Tube](https://www.sigmaaldrich.com/CH/de/product/supelco/57088) Schnellansicht [ \ Supelco \ 57094 \ Supelclean™ ENVI-Carb™ SPE Tube](https://www.sigmaaldrich.com/CH/de/product/supelco/57094) Schnellansicht [ \ Supelco \ 52738-U \ Supelclean™ PSA SPE Bulk Packing](https://www.sigmaaldrich.com/CH/de/product/supelco/52738u) Schnellansicht [ \ Supelco \ 57210-U \ Supelclean™ ENVI-Carb™ SPE Bulk Packing](https://www.sigmaaldrich.com/CH/de/product/supelco/57210u) Schnellansicht [ \ Supelco \ 28399-U \ Dual-Layer Carbon Reversible Tube (Micro-Column)](https://www.sigmaaldrich.com/CH/de/product/supelco/28399u) Schnellansicht [ \ Supelco \ 28397-U \ Multi-Layer Silica Gel Dioxin Column](https://www.sigmaaldrich.com/CH/de/product/supelco/28397u) Schnellansicht [ \ Supelco \ 57044 \ Visiprep™ SPE Vacuum Manifold](https://www.sigmaaldrich.com/CH/de/product/supelco/57044) Schnellansicht [ \ Supelco \ 57265 \ Visiprep™ SPE Vacuum Manifold](https://www.sigmaaldrich.com/CH/de/product/supelco/57265) Schnellansicht [ \ Supelco \ 57493-U \ Supel™ Swift HLB SPE Tubes](https://www.sigmaaldrich.com/CH/de/product/supelco/57493u) Schnellansicht [ \ Supelco \ 55274-U \ Supel™Genie C8 Online SPE Starter Kit](https://www.sigmaaldrich.com/CH/de/product/supelco/55274u) Schnellansicht [ \ Supelco \ 55228-U \ Supel™ QuE](https://www.sigmaaldrich.com/CH/de/product/supelco/55228u) Schnellansicht [ \ Supelco \ 55227-U \ Supel™ QuE](https://www.sigmaaldrich.com/CH/de/product/supelco/55227u) Schnellansicht [ \ Supelco \ 52977-U \ HybridSPE® DPX Tip](https://www.sigmaaldrich.com/CH/de/product/supelco/52977u) Schnellansicht [ \ Supelco \ 575608-U \ Discovery® DSC-Si SPE 96-well Plate](https://www.sigmaaldrich.com/CH/de/product/supelco/575608u) Schnellansicht [ \ Supelco \ 54341-U \ Supelclean™ EZ-POP NP](https://www.sigmaaldrich.com/CH/de/product/supelco/54341u) Schnellansicht [ \ Supelco \ 55324-U \ Supel™Genie HybridSPE® Online SPE Starter Kit](https://www.sigmaaldrich.com/CH/de/product/supelco/55324u) Schnellansicht [ \ Supelco \ 52974-U \ HybridSPE® DPX Tip](https://www.sigmaaldrich.com/CH/de/product/supelco/52974u) Schnellansicht * * * ## Besondere Kategorien [](https://www.sigmaaldrich.com/CH/de/products/analytical-chemistry/analytical-sample-preparation/spe-and-quechers) [Festphasenextraktion (SPE) und QuEChERS](https://www.sigmaaldrich.com/CH/de/products/analytical-chemistry/analytical-sample-preparation/spe-and-quechers) Entdecken Sie verschiedene Materialien für die Festphasenextraktion (SPE) und QuEChERS-Probenvorbereitung. Kartuschen, Röhrchen, Platten und mehr verfügbar. [Produkte einkaufen](https://www.sigmaaldrich.com/CH/de/products/analytical-chemistry/analytical-sample-preparation/spe-and-quechers) [](https://www.sigmaaldrich.com/CH/de/products/analytical-chemistry/analytical-chromatography/hplc-columns) [HPLC-Säulen](https://www.sigmaaldrich.com/CH/de/products/analytical-chemistry/analytical-chromatography/hplc-columns) Erzielen Sie präzise Trennungen mit unserer umfangreichen HPLC-Säulensammlung. Verbessern Sie Retention, Auflösung und Selektivität. Bestellen Sie noch heute. [Produkte einkaufen](https://www.sigmaaldrich.com/CH/de/products/analytical-chemistry/analytical-chromatography/hplc-columns) [](https://www.sigmaaldrich.com/CH/de/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) [GC-Säulen, Zubehör und Adsorbentien](https://www.sigmaaldrich.com/CH/de/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) Erzielen Sie eine hervorragende GC-Leistung mit unseren Säulen, Verbrauchsmaterialien und Zubehör. Entdecken Sie das Angebot von Supelco®. [Produkte einkaufen](https://www.sigmaaldrich.com/CH/de/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) * * * Übersicht Verwandte Artikel und Protokolle Unterstützung Die Festphasenextraktion (SPE) ist eine Technik zur schnellen, selektiven Probenvorbereitung und -aufreinigung vor der chromatographischen Analyse (z. B. HPLC, GC, TLC). Bei der SPE werden ein oder mehrere Analyten aus einer flüssigen Probe durch Extraktion, Partitionierung und/oder Adsorption an eine feste stationäre Phase isoliert. Dadurch wird die Probe für die anschließende analytische Chromatographie besser geeignet, was die endgültige qualitative und quantitative Analyse oft vereinfacht und verbessert. Durch die einfachere Probenmatrix werden auch die Anforderungen an ein Analysesystem verringert, wodurch sich die Lebensdauer des Systems verlängern kann. Ein optimales SPE-Verfahren kann Folgendes ermöglichen: - Die Probenmatrix wird so verändert, dass sie mit der angestrebten chromatographischen Methode besser kompatibel ist. - Konzentration von Analyten (Spurenanreicherung) zur Erhöhung der Empfindlichkeit. - Entfernen von Interferenzen, die einen hohen Hintergrund, irreführende Peaks und/oder schlechte Empfindlichkeit während der chromatographischen Analyse verursachen. - Schützen Sie die analytische Säule vor Verunreinigungen. - Automatisieren Sie den Extraktionsprozess. ## Wie funktioniert SPE? Bei der SPE bindet die stationäre Phase (ein Sorptionsmittel oder Harz) entweder den Analyten oder die Verunreinigung durch starke, aber reversible Wechselwirkungen, um den interessierenden Analyten zuverlässig und schnell aus einer komplexen Probe zu extrahieren. Die SPE ist selektiv und vielseitig, da viele verschiedene Sorptionsmittel und Elutionsbedingungen für unterschiedliche Analyten und Matrices verfügbar sind. Zu den gängigen SPE-Sorbentien gehören: - Silika-basiert - Umgekehrte Phase (C18, C8, Cyan, Phenyl) - Normale Phase (Kieselsäure, Diol, NH2) - Ionenaustausch (SAX, WCX, SCX) - Kohlenstoff-basiert - Polymer-basiert (verschiedene Zusammensetzungen, verschiedene Funktionalitäten) - Andere, zum Beispiel Florisil® (Magnesiumsilikat) oder Aluminiumoxid - Mischbett: Kombinationen aus nahezu allen oben genannten Materialien in aufeinanderfolgenden Schichten ## Strategien für die SPE Bei der 'Bind-Elute SPE' wird der interessierende Analyt durch das Sorptionsmittel eingefangen, während die Matrixinterferenzen die Kartusche passieren. Bei der 'Interference Removal SPE' werden die Matrix-Interferenzen auf dem Sorptionsmittel eingefangen und die Analyten von Interesse passieren die Kartusche. Sowohl HybridSPE als auch [](https://www.sigmaaldrich.com)[QuEChERS](https://www.sigmaaldrich.com/CH/de/applications/analytical-chemistry/sample-preparation/quechers) SPE-Methoden funktionieren über Interference Removal. Die optimale SPE-Methode hängt von der Struktur, der Löslichkeit, der Polarität und den lipophilen Eigenschaften (Verteilungskoeffizienten) des Analyten ab. [](https://www.sigmaaldrich.com)Auswahlhilfen helfen bei der Auswahl der günstigsten stationären Phase und des Lösungsmittels für die jeweilige Anwendung. ## Gängige Anwendungen der SPE SPE wird häufig in der pharmazeutischen, klinischen und Hochdurchsatzdiagnostik, in der forensischen, umwelttechnischen und lebensmittel- bzw. agrochemischen Industrie für Analysen eingesetzt, die mit folgenden Themen in Zusammenhang stehen: - Pharmazeutische Verbindungen und Metaboliten in biologischen Flüssigkeiten - Drogenmissbrauch in biologischen Flüssigkeiten - Umweltschadstoffe in Trink- und Abwässern - Pestizide, Antibiotika oder Mykotoxine in Lebensmitteln/landwirtschaftlichen Matrices - Entsalzung von Proteinen und Peptiden - Fraktionierung von Lipiden - Wasser- und fettlösliche Vitamine [](https://www.sigmaaldrich.com/documents-search) [Suchen Sie nach spezifischeren Informationen?](https://www.sigmaaldrich.com/documents-search) Besuchen Sie unsere Dokumentensuche für Datenblätter, Zertifikate und technische Dokumentation. [Dokumente finden](https://www.sigmaaldrich.com/documents-search) ## Verwandte Artikel - [Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC](https://www.sigmaaldrich.com/CH/en/technical-documents/protocol/environmental-testing-and-industrial-hygiene/air-testing/aldehydes-air-dnph) Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection. - [Effect of Container Materials on Milk Off-Flavors](https://www.sigmaaldrich.com/CH/en/technical-documents/technical-article/analytical-chemistry/gas-chromatography/container-materials) HS-SPME with Carboxen®/PDMS coated Nitinol fiber allows low MW analyte detection in light-exposed milk plastics. - [Pesticide Residue Analysis in Ginger Powder with QuEChERS](https://www.sigmaaldrich.com/CH/en/technical-documents/technical-article/analytical-chemistry/quechers/analysis-of-pesticide-residues-in-ginger-powder) 2-layer SPE cartridge efficiently reduces background for ginger contaminant analysis. - [Analysis of Pesticide Residues in Pistachios Using QuEChERS Extraction and Cleanup with Supel™ QuE Z-Sep+](https://www.sigmaaldrich.com/CH/en/technical-documents/technical-article/analytical-chemistry/quechers/analysis-of-pesticide-residues-in-pistachios) Pistachios 45% fat complicates pesticide analysis. But Z-Sep+ sorbent cleanup after QuEChERS extraction can reduce background for reliable results. - [HybridSPE-Phospholipid Technology](https://www.sigmaaldrich.com/CH/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/hybridspe-precipitation-technology) This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed. - [Alle sehen (41)](https://www.sigmaaldrich.com/CH/de/search/facet-search?focus=sitecontent&term=facet-search) ## Verwandte Protokolle - [Bestimmung von Bisphenol A in Trinkwasser](https://www.sigmaaldrich.com/CH/de/technical-documents/protocol/environmental-testing-and-industrial-hygiene/drinking-water-testing/bisphenol-a-water) Supelco hat ein umfassendes Probenvorbereitungs- und Analyseverfahren für die Bestimmung von Bisphenol A (BPA) in Trinkwasser entwickelt. - [Bestimmung von Triclosan in Gewässern](https://www.sigmaaldrich.com/CH/de/technical-documents/protocol/environmental-testing-and-industrial-hygiene/waste-water-and-process-water-testing/determination-triclosan-water) Triclosan wurde in der Ableitung einer Kläranlage mittels HPLC und anschließendem UV- und MS/MS-Nachweis ermittelt. - [Empowering Biochemists to Analyze Glycoproteins with Exceptional Reproducibility](https://www.sigmaaldrich.com/CH/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/bioshell-glycan-hplc-columns) Fast, high-resolution separations of procainamide labeled glycans - dextran ladder, human IgG and cetuximab - [Biological Testing for Amphetamine and Methamphetamine Abuse](https://www.sigmaaldrich.com/CH/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/biological-testing-for-amphetamine-and-methamphetamine-abuse) In this study, optimized methods are presented for sample preparation and chiral chromatography for the LC/MS analysis of amphetamine and methamphetamine enantiomers in urine. - [Accurate Biological Testing for Amphetamine and Methamphetamine Abuse Using Chiral HPLC and MS Detection](https://www.sigmaaldrich.com/CH/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/chiral-hplc-amphetamine-methamphetamine) Optimized sample prep and chiral chromatography methods for the LC/MS analysis of these drug enantiomers in urine - [Alle sehen (34)](https://www.sigmaaldrich.com/CH/de/search/facet-search?focus=sitecontent&term=facet-search) ### Weitere Artikel und Protokolle finden Schlüsselwörter eingeben Suche ## Wie können wir helfen Bei Fragen stellen Sie bitte eine [Kundensupportanfrage](https://www.sigmaaldrich.com/CH/de/support/customer-support) oder sprechen Sie mit unserem Kundenservice: E-Mail [[email protected]](mailto:[email protected]) oder rufen Sie an unter +1 (800) 244-1173 ## Zusätzliche Unterstützung - [Chromatogram Search](https://www.sigmaaldrich.com/chromatogram-search) Use the Chromatogram Search to identify unknown compounds in your sample. - [Rechner & Apps](https://www.sigmaaldrich.com/CH/de/support/calculators-and-apps) Web-Toolbox - wissenschaftliche Forschungstools und Informationsquellen für die Bereiche analytische Chemie, Life Science, chemische Synthese und Materialwissenschaft. - [Customer Support Request](https://www.sigmaaldrich.com/CH/de/support/customer-support) Customer support including help with orders, products, accounts, and website technical issues. - [FAQ](https://maestro.my.site.com/knowledgeportal/s/) Explore our Frequently Asked Questions for answers to commonly asked questions about our products and services. * * * __Ausgewählte Artikel__ [Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC](https://www.sigmaaldrich.com/CH/en/technical-documents/protocol/environmental-testing-and-industrial-hygiene/air-testing/aldehydes-air-dnph) Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection. [Effect of Container Materials on Milk Off-Flavors](https://www.sigmaaldrich.com/CH/en/technical-documents/technical-article/analytical-chemistry/gas-chromatography/container-materials) HS-SPME with Carboxen®/PDMS coated Nitinol fiber allows low MW analyte detection in light-exposed milk plastics. [Pesticide Residue Analysis in Ginger Powder with QuEChERS](https://www.sigmaaldrich.com/CH/en/technical-documents/technical-article/analytical-chemistry/quechers/analysis-of-pesticide-residues-in-ginger-powder) 2-layer SPE cartridge efficiently reduces background for ginger contaminant analysis. [HybridSPE-Phospholipid Technology](https://www.sigmaaldrich.com/CH/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/hybridspe-precipitation-technology) This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed. 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