Merck
All Photos(1)

G0924

Millipore

Glutathione High Capacity Magnetic Agarose Beads

(1:1 suspension in a 30% ethanol solution)

MDL-Nummer:
NACRES:
NA.56

Konjugat

magnetic beads

Qualitätsniveau

200

Form

(1:1 suspension in a 30% ethanol solution)

analyte chemical class(es)

proteins (GST)

technique(s)

immunoprecipitation (IP): suitable
protein purification: suitable

Matrix

4% beaded magnetic agarose

Matrix-Spacer

12 atoms

Kapazität

≥15 μmol/mL, agarose binding capacity

versandt in

wet ice

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Glutathione Magnetic Agarose Beads consist of a paramagnetic 4% beaded agarose. Glutathione is covalently attached through the sulfur to epoxy-activated agarose resulting in a 12 atom (10 carbon) spacer.

The Glutathione Magnetic Agarose Beads are useful for affinity capture, molecular pull-down, or immunoprecipitation (IP) of proteins containing glutathione binding sequences, such as native glutathione S-transferase (GST), glutathione peroxidase, and glyoxalase I, or GST-tagged recombinant fusion proteins from cell lysates or other biochemical solutions while exhibiting low non-specific binding of other proteins. Recombinant GST-tagged proteins, bound to the affinity resin are separated with the use of a magnet. The magnetic properties allow for very rapid processing, as well as aid in manipulations, such as repetitive washings and recovery of the desired protein. This leads to faster recovery, experimental reproducibility, and more accurate quantitation of the proteins of interest.

The matrix of the magnetic beads is a 4% beaded agarose with an average diameter of 50 μ and a diameter range of 20-75 μ. Paramagnetic iron is impregnated within the beads.

Anwendung

Useful for affinity capture, molecular pull-down, or immunoprecipitation (IP) of proteins containing glutathione binding sequences, such as recombinant GST-tagged fusion proteins or native glutathione S-transferase, glutathione peroxidase and glyoxalase I from cell lysates and other biological samples. Can be used for purification in single tubes or for high throughput in 96-well multiwell plates. The magnetic properties of the beads aid in manipulations, such as repetitive washings and recovery of the protein. This leads to greater experimental reproducibility and more accurate quantitation of the proteins of interest.

Physikalische Form

1:1 suspension in a 30% ethanol solution

Piktogramme

Flame

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Flam. Liq. 2

Lagerklassenschlüssel

3 - Flammable liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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