Merck
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H9914

Millipore

HIS-Select® Nickel Magnetic Agarose Beads

Synonym(s):
nickel charged magnetic beaded agarose
NACRES:
NA.56

Konjugat

magnetic beads

Qualitätsniveau

200

Form

suspension

Haltbarkeit

2 yr (Unopened product)

Matrix

6% beaded magnetic agarose

Kapazität

≥15 mg/mL binding capacity

Lagertemp.

2-8°C

Allgemeine Beschreibung

HIS-Select Magnetic Agarose Beads consist of paramagnetic, immobilized metal-ion affinity chromatography (IMAC) resin that contain a proprietary quadridentate chelate, which is bound with nickel and covalently attached through a non-charged, hydrophilic linker to magnetic beaded agarose. The magnetic properties of the beads aid in manipulations, such as repetitive washings, and recovery of the protein bound beads. This leads to greater experimental reproducibility and more accurate quantitation of the His-tagged proteins of interest.

Anwendung

The HIS-Select Nickel Magnetic Agarose Beads are designed for use in automated and small-scale affinity capture (molecular pull-down) purifications of histidine-tagged protein / His-tag protein while exhibiting low non-specific binding of other proteins. The beads can be used to purify His-tagged proteins under native and denaturing conditions.

Physikalische Form

Supplied as a 50% slurry suspension in 30% Ethanol.

Rechtliche Hinweise

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC

Signalwort

Danger

Gefahreneinstufungen

Aquatic Chronic 3 - Carc. 1A - Flam. Liq. 3 - Repr. 1B - Skin Sens. 1 - STOT RE 2

Zielorgane

Respiratory Tract

Lagerklassenschlüssel

3 - Flammable liquids

WGK

WGK 3

Flammpunkt F

86.0 °F

Flammpunkt C

30 °C

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Certificate of Origin

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