HomeWebinarsProtein Sample Prep Tips & Tricks: Improving protein yield and purity from Recombinant Bacterial Expression

Protein Sample Prep Tips & Tricks: Improving protein yield and purity from Recombinant Bacterial Expression


E. coli is the most widely used recombinant expression system to overexpress protein given that it is inexpensive, easy to scale up, and relatively fast. Due to its wide-spread use, there are numerous molecular tools, products, and expression/purification protocols available. Determining which tools and products to use, such as plasmid, strain-type, affinity tag and resin system, or buffer exchange device can be daunting. Here, we present a workflow overview of the recombinant protein expression from E. coli and provide insight and various tips and tricks about how to optimize and improve protein yield and purity enabling you to make the best decisions for your protein of interest.

What Will You Learn?

Determining best conditions for:

  • Protein overexpression in E. coli
  • Affinity protein purification
  • Protein concentration and buffer exchange
  • Additional tips about membrane ultrafiltration fractionation, and detergent removal

Who Should Watch?

Scientists, lab technicians, researchers who work with protein


Natasha L. Pirman, Ph.D

Molecular Workflow Tools

Lead Application Scientist

Dr. Natasha L. Pirman is an Application Scientist for Molecular Workflow Tools where she is involved in developing new sample preparation products and applications for both protein and nucleic acid applications. Prior to joining us, Dr. Pirman utilized her expertise in protein biochemistry to explore structural protein changes to Intrinsically Disordered Proteins and human kinase disease models for drug target investigations.

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.