Merck
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1.11723

Millipore

NutriSelect® plus Casein-peptone lecithin polysorbate broth (base)

USP, For sterility tests of viscous or gelatinous materials

Synonym(s):
TAT broth

Agency

USP

Quality Level

form

powder

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

application(s)

cosmetics
microbiology
pharmaceutical

storage temp.

15-25°C

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This Item
1.058781.04316P0514
agency

USP

agency

ISO 11133, according to ISO 6579

agency

EP, ISO 21528, ISO 22964, ISO 6579, ISO 6887

agency

-

form

powder

form

medium granules (dehydrated (DCM))

form

liquid

form

solution

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

GranuCult®

manufacturer/tradename

NutriSelect® Prime

manufacturer/tradename

-

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

-

application(s)

cosmetics
microbiology
pharmaceutical

application(s)

food and beverages
microbiology

application(s)

food and beverages

application(s)

-

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

−20°C

General description

Casein-peptone lecithin polysorbate broth (base) is a granulated medium recommended for general microbiological analysis. The granules ensure rapid and uniform dissolution in water, prevent clumping of the medium, and inhalation of the airborne powder. Casein enzymic hydrolysate provides the nitrogen, vitamins, amino acids, and carbon in the broth base. Casein peptone in the medium present optimal conditions for the spore germination and regeneration of the damaged microorganisms. Lecithin and polysorbate 20 inactivate many antimicrobial compounds.

Application

Casein-peptone lecithin polysorbate broth (base) is recommended for diluting samples of pharmaceutical, cosmetic, and other raw materials or final products for determining the total microbial counts.

Analysis Note

Appearance (clearness): clear to almost clear
Appearance (colour): yellowish
pH-value (25 °C): 6.9 - 7.3
Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032)): +
Growth (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)): +
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003)): +
Growth (Candida albicans ATCC 10231 (WDCM 00054)): +
Growth (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): +
Incubation: 24 hrs.; 35 °C; aerobic;
Aspergillus und C.albicans up to 3 days; 20 - 25 °C; aerobic .

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

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