Documents

17-295

Sigma-Aldrich

Chromatin Immunoprecipitation (ChIP) Assay Kit

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

Synonym(s):
Agarose ChIP Kit
eCl@ss:
32161000
NACRES:
NA.41

Quality Level

manufacturer/tradename

EZ-ChIP

application(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

Related Categories

General description

For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.

Packaging

Kit capacity: 22 Assays

Components

Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)

All necessary buffers

Quality

Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.

Physical form

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

pictograms

Exclamation mark

signalword

Warning

storage_class_code

10-13 - German Storage Class 10 to 13

Certificate of Analysis

Certificate of Origin

Regulation of human papillomavirus type 31 gene expression during the differentiation-dependent life cycle through histone modifications and transcription factor binding.
Tonia R Wooldridge, Laimonis A Laimins
Virology null
EZH2-dependent suppression of a cellular senescence phenotype in melanoma cells by inhibition of p21/CDKN1A expression.
Tao Fan,Shunlin Jiang,Nancy Chung,Ali Alikhan,Christina Ni,Chyi-Chia Richard Lee,Thomas J Hornyak
Molecular Cancer Research null
Chromatin immunoprecipitation for identifying transcription factor targets in keratinocytes.
Kori Ortt,Satrajit Sinha
Methods in Molecular Biology null
D Pannell et al.
The EMBO journal, 19(21), 5884-5894 (2000-11-04)
Retrovirus vectors are de novo methylated and transcriptionally silent in mammalian stem cells. Here, we identify epigenetic modifications that mark retrovirus-silenced transgenes. We show that murine stem cell virus (MSCV) and human immunodeficiency virus type 1 (HIV-1) vectors dominantly silence...
A novel cis-acting element in Her2 promoter regulated by Stat3 in mammary cancer cells
Qian, Lu, et al
Biochemical and biophysical research communications null
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