Radioimmunoprecipitation assay (RIPA) Buffer aids in cell lysis and solubilization of proteins from both adherent and suspension-cultured mammalian cells. In addition, this buffer helps in relevant protein-protein interactions studies by reducing non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur.
RIPA Lysis Buffer, 10X has been used for cell lysis during the sample preparation for immunoprecipitation and western blot.
0.5M Tris-HCl, pH 7.4, 1.5M NaCl, 2.5% deoxycholic acid, 10% NP-40, 10mM EDTA.
Storage and Stability
6 months at room temperature
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