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206700

Sigma-Aldrich

Calcein-AM

A cell-permeable, non-fluorescent, and hydrophobic compound, which is rapidly hydrolyzed by intracellular esterases releasing the membrane-impermeable, hydrophilic, and intensely fluorescent calcein.

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Synonym(s):
Calcein-AM, Calcein-acetoxymethyl Ester, Diacetate, CAL-AM
Empirical Formula (Hill Notation):
C46H46N2O23
CAS Number:
Molecular Weight:
994.86
MDL number:

Quality Level

Assay

≥90% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

off-white

solubility

DMSO: 5 mg/mL

fluorescence

λex 494 nm
λem 517 nm

shipped in

ambient

storage temp.

−20°C

InChI

1S/C46H46N2O23/c1-25(49)60-21-64-41(55)17-47(18-42(56)65-22-61-26(2)50)15-31-11-35-39(13-37(31)68-29(5)53)70-40-14-38(69-30(6)54)32(12-36(40)46(35)34-10-8-7-9-33(34)45(59)71-46)16-48(19-43(57)66-23-62-27(3)51)20-44(58)67-24-63-28(4)52/h7-14H,15-24H2,1-6H3

InChI key

BQRGNLJZBFXNCZ-UHFFFAOYSA-N

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This Item
1778356496208773
Calcein-AM A cell-permeable, non-fluorescent, and hydrophobic compound, which is rapidly hydrolyzed by intracellular esterases releasing the membrane-impermeable, hydrophilic, and intensely fluorescent calcein.

Sigma-Aldrich

206700

Calcein-AM

Calcein-AM BioReagent, suitable for fluorescence, ≥95.0% (HPLC)

Sigma-Aldrich

17783

Calcein-AM

Calcein-AM Small Package (20 X 50 μg ), ≥95.0% (HPLC)

Sigma-Aldrich

56496

Calcein-AM

form

solid

form

-

form

-

form

lyophilized solid

color

off-white

color

-

color

-

color

-

solubility

DMSO: 5 mg/mL

solubility

-

solubility

-

solubility

DMSO: 5 mg/mL

fluorescence

λex 494 nm, λem 517 nm

fluorescence

λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+

fluorescence

λex 496 nm; λem 516 nm±5 nm in 0.1 M Tris pH 8.0, esterase; Ca2+

fluorescence

λex 480 nm; λem 320 nm

shipped in

ambient

shipped in

-

shipped in

-

shipped in

ambient

General description

A cell-permeable, non-fluorescent, and hydrophobic compound, which is rapidly hydrolyzed by intracellular esterases releasing the membrane-impermeable, hydrophilic, and intensely fluorescent calcein. Can also be used for imaging mitochondrial permeability pore transition. Calcein is better retained by viable cells than fluorescein, carboxyfluorescein, or BCECF, and its fluorescence is brighter in a variety of mammalian cell types. Useful for assessing adhesion, cell viability, chemotaxis, and multidrug resistance (MDR).
A cell-permeable, nonfluorescent, and hydrophobic compound, which is rapidly hydrolyzed by cytoplasmic esterases inside the cell, releasing the membrane-impermeable, hydrophilic, and intensely fluorescent calcein (excitation maximum: 494 nm; emission maximum: 517 nm). A cytosolic fluorophore that can also be used imaging mitochondrial permeability pore transition. Calcein is better retained by viable cells than fluorescein, carboxyfluorescein, and BCECF and its fluorescence is brighter in a variety of mammalian cell types. Useful for assessing adhesion, cell viability, chemotaxis and multidrug resistance (MDR).

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Imaging mitochondrial permeability pore transition
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.

Other Notes

Dhar, S., et al. 1998. Eur. J. Pharmacol. 346, 315.
Essodaigui, M., et al. 1998. Biochemistry 37, 2243.
Lemasters, J.J., et al. 1998. Biochim. Biophys. Acta 1366, 177.
Feller, N., et al. 1995. Br. J. Cancer 72, 543.
Feller, N., et al. 1995. FEBS Lett. 368, 385.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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