Research Sub Category
Infectious Diseases - Viral
Pan-Enterovirus Blend (Catalog No. 3360)
1. One 5 mL dropper vial containing a blend of two mouse monoclonal antibodies which recognize all enteroviruses including echoviruses, coxsackie A and B viruses, polio 1,2, and 3, and enteroviruses 70 and 71, protein stabilizer and 0.1% sodium azide (preservative). The Pan-Enterovirus Blend may cross-react with hepatitis A, reovirus 3, astrovirus and rhinovirus.
2. Pan-Enterovirus Control Slides - (Catalog No. 5069). Two control slides containing five infected (positive) wells and one non-infected (negative) wells. Each infected well represents a different enterovirus: Coxsackievirus A9, coxsackievirus B2, echovirus 30, enterovirus 71 and poliovirus 2. There is one uninfected (negative) well.
3. Normal Mouse Antibody – (Catalog No. 5014). One 10 ml dropper vial containing normal mouse antibody to be used as a negative control.
4. Anti-Mouse IgG:FITC – (Catalog No. 5008). One 10 ml dropper vials containing FITC labeled goat anti mouse IgG.
5. Phosphate Buffered Saline - (Catalog No. 5087). One packet of Phosphate Buffered Saline Salts yields 1 liter when dissolved in distilled water. Store in a clean closed container at room temperature.
6. Tween 20 / Sodium Azide Solution (100X) - (Catalog No. 5037). One 10 ml vial containing polyoxyethylene sorbitan monolaurate (Tween 20) and sodium azide (NaN3) concentrate to be diluted 1:100 in PBS.
7. Mounting Fluid - (Catalog No. 5013). One 10 mL dropper vial containing Tris-buffered glycerin, a fluorescence enhancer, and 0.1% sodium azide (preservative).
Storage and Stability
When stored at 2-8°C, the Pan-Enterovirus Blend is stable up to the expiration date printed on the label. Do not freeze or expose to elevated temperatures. Discard any remaining reagent after the expiration date.
Warning and Precautions:
· Incubation times or temperatures other than those specified may give erroneous results. Any change must be validated by the user.
* The performance of the Light Diagnostics Pan-Enterovirus Blend has not been determined on direct specimens.
· The monoclonal antibodies present in the Pan-Enterovirus Detection Kit may show some background in certain cell lines, such as HEp-2 cells or some monkey cell lines.
· Slides prepared too early (<25% CPE) or too late (>95% CPE) can be difficult to read and can lead to false negative results.
· Sodium azide, present in the reagent, may react with lead and copper plumbing to form potentially explosive metal azides. Upon disposing of solutions that contain sodium azide, flush plumbing with a large volume of water to prevent build-up.
* Handle all specimens and materials coming in contact with them as potentially infectious materials. Decontaminate with 0.05% sodium hypochlorite prior to disposal.
* Avoid contact with Evans blue, if present in any reagent, as it is a potential carcinogen. If skin contact occurs, flush with large volumes of water.
* Do not mouth pipette reagents.
· Do not allow shell vials or slides to dry at any time during the staining procedure.
· Pooling or alteration of any reagent may cause erroneous results.
· Acetone is extremely flammable and harmful if swallowed or inhaled. Keep away from heat, sparks or flame. Avoid breathing vapor. Use adequate ventilation.
· Mounting Fluid (Catalog No. 5013) contains a fluorescence enhancer that may be destructive to mucous membranes. Avoid direct skin or mucous membrane contact. If contact occurs, flush with large volumes of water.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
For in vitro Diagnostic Use