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475904-M

Millipore

MOWIOL® 4-88 Reagent

Synonym(s):
Poly(vinyl alcohol)
Linear Formula:
[-CH2CHOH-]n
CAS Number:
MDL number:

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

yellow

storage temp.

10-30°C

InChI

1S/C2H4O/c1-2-3/h2-3H,1H2

InChI key

IMROMDMJAWUWLK-UHFFFAOYSA-N

General description

Used for mounting cells on slides for detection of cell-surface antigens by immunofluorescence. Useful as an anti-fading agent.

Warning

Toxicity: Standard Handling (A)

Preparation Note

Add 2.4 g of MOWIOL® 4-88 to 6 g of glycerol and stir to mix. Add 6 ml of water and stir for several hours at room temperature. Add 12 ml of 0.2 M Tris (pH 8.5) and heat to 50°C for 10 min. with occasional mixing. After the MOWIOL 4-88 dissolves, clarify the solution by centrifugation at 5000 x g for 15 min.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 weeks at 4°C.

Other Notes

Longin, A., et al. 1993. J. Histochem. Cytochem. 41, 1833.
Beug, H., et al. 1979. Cell18, 375.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Mowiol is a registered trademark of Kuraray Europe GmbH

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Certificate of Analysis

Certificate of Origin

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