Merck
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524604

Sigma-Aldrich

Phosphatase, Alkaline, Human Placenta

Synonym(s):
Phosphatase, Alkaline, Human Placenta
CAS Number:
Enzyme Commission number:
MDL number:

form

liquid

Quality Level

specific activity

≥100 U/mg
≥300 units/mL

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

shipped in

wet ice

storage temp.

2-8°C

General description

Native alkaline phosphatase from human placenta.
Native alkaline phosphatase from human placenta. Homodimeric enzyme that hydrolyzes phosphate esters of primary and secondary alcohols, amines, and phenols. Useful in the hydrolysis of 5′-terminal phosphates of DNA and RNA. Active site contains a reactive serine.

Packaging

100 u in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 μmol pNPP per min at 37°C, pH 10.15.

Physical form

In 10 mM Tris-HCl, 1 mM MgCl₂, 0.1 mM ZnCl₂, 50% glycerol, pH 7.6.

Preparation Note

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Other Notes

Fishman, W.H., et al. 1973. Anal. Biochem.51, 368.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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