Merck
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CB1001

Sigma-Aldrich

Anti-GAPDH Mouse mAb (6C5)

liquid, clone 6C5, Calbiochem®

Synonym(s):
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

6C5, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

fish, chicken, frog, porcine, canine, mouse, rabbit, human, rat

should not react with

yeast, bovine

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... GAPDH(2597)
mouse ... Gapdh(14433)
rabbit ... Gapdh(100009074)

General description

Anti-GAPDH, mouse monoclonal, clone 6C5, recognizes ~146 kDa GAPDH protein under non-reducing conditions & 36 kDa monomers under reducing conditions. It is validated for use in ELISA, WB, and IHC.
Protein A purified mouse monoclonal antibody. Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.
Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.

Immunogen

Rabbit
purified, rabbit muscle

Packaging

500 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Application


Warning

Toxicity: Highly Toxic (H)

Physical form

In PBS, pH 7.4.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.

Analysis Note

Positive Control
Rat skeletal muscle

Other Notes

Useful as a loading or positive control since GAPDH is expressed in all cells. Also reported to work for ELISA and immunocytochemistry. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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