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EZGLP1T

Sigma-Aldrich

Multi Species GLP-1 Total ELISA, EZGLP1T-36K

This Multi Species GLP-1 Total ELISA, EZGLP1T-36K, is used to measure & quantify Glucagon Like Peptide-1 levels in Metabolism research.

eCl@ss:
32161000
NACRES:
NA.32

Quality Level

species reactivity

human, rat, mouse

assay range

linearity: 90-132%
recovery range: 90-110%
sensitivity: 1.5 pM
(MinDC)

standard curve range: 4.1-1000 pM
inter-assay cv: <12%
intra-assay cv: <5%

application(s)

ELISA: suitable

NCBI accession no.

UniProt accession no.

Gene Information

human ... GCG(2641)

General description

Glucagon-like peptide-1 (GLP-1) is a 30 amino acid gut hormone secreted from the intestinal L-cells. The GLP-1 sequence is highly conserved among mammals and is released in its active form (7-36) in response to food intake. It is quickly degraded into its inactive form (9-36) after cleavage by DPPIV. GLP-1 reduces glucose levels by regulating pancreatic secretion, slowing gastric emptying and lowering the desire for food intake. Therefore, it is an important biomarker for the study of diabetes and obesity. This kit measures both the active (7-36) and inactive (9-36) forms of GLP-1.

Specificity

Cross Reactivty
GLP-1 is conserved in almost all mammals.
The antibody pair used in this assay is specific to GLP-1 (7-36) and (9-36) and has no cross-reactivity with GLP-2, GIP, Glucagon or Oxyntomodulin.

Application

Used to detect/quantify: Glucagon Like Peptide-1
This is a 3.5-hour assay at room temperature requiring 50 uL of serum, plasma or culture samples per well.
Research Category
Metabolism

Disclaimer

For research use only. Not for use in diagnostic procedures.

Certificate of Analysis

Certificate of Origin

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