GF003AF
Fibroblast Growth Factor basic, human recombinant, animal-free
The Fibroblast Growth Factor-basic (or bFGF protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.
Synonym(s):
FGF-2, bFGF, basic-FGF, Heparin-Binding Growth Factor 2, Prostatropin
About This Item
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biological source
human
Quality Level
Assay
>95% (SDS-PAGE)
manufacturer/tradename
Chemicon®
technique(s)
cell culture | stem cell: suitable
impurities
<0.1 EU/μg Endotoxin (of bFGF)
input
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
NCBI accession no.
UniProt accession no.
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General description
Application
Physical form
Storage and Stability
General applications:
After a quick spin, reconstitute in 0.1M phosphate buffer, pH 6.8, to a concentration of 0.1-1.0 mg/mL. Reconstituted bFGF should be stored in working aliquots at -20°C for up to six months. Multiple freeze/thaw cycles will result in significant loss of activity.
For Human ES cell culture:
After a quick spin, reconstitute to 10 μg/mL in a filtered solution of 0.5% BSA, 1 mM DTT, and 10% glycerol in Dulbecco′s PBS. Aliquot and store at -20°C for up to six months. This solution can then thawed and diluted to 4 ng/mL for the culture of human ES cells with a feeder layer, or to 8 ng/mL to supplement mouse embryonic fibroblast-conditioned medium (for feeder-free human ES cell culture).
Analysis Note
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WGK
WGK 3
Certificates of Analysis (COA)
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