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HX0514

Sigma-Aldrich

Hyaluronidase

Synonym(s):

Hyaluronidase

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.78

conjugate

conjugate (Glucosaminoglycan)

Quality Level

form

powder

specific activity

≥300 units/mg dry wt.

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

shipped in

ambient

storage temp.

−20°C

General description

300 units/mg minimum, 50,000 units/vial. Suitable for biochemistry grade.

Specifications:Activity: 300NF U/mg min





Intended for laboratory and manufacturing use only. Not for drug, food, or household use.

Application

Hyaluronidase has been used:
  • to enzymatically remove extra-cellular matrix (ECM) to assess the lateral diffusion of the synaptic a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR)
  • to study the effects of 4-methylumbelliferone (4MU)/ hyaluronidase (HLX)-mediated depletion of the CD44/hyaluronan complex on trastuzumab binding in JIMT1 breast cancer xenografts
  • as a component of Hank′s balanced salt solution (HBSS) medium to digest fetal intestine tissue

Biochem/physiol Actions

Hyaluronidase is an endoglycosidase that degrades hyaluronic acid (HA), a component of the extracellular matrix. It is used to enhance drug diffusion and reverse the effects of HA filler injections. Hyaluronidases dissolve the subcutaneous nodules and can correct excessive quantities of injected filler. It eliminates the cumulus-corona-oocyte complex formed during intracytoplasmic sperm injection. Hyaluronidase inhibits tissue damage after the extravasation of several substances. It is used for reducing edema treating vitreous hemorrhage.

Unit Definition

One unit is based on the change in absorbency (turbidity) at 540 nm of an internal standard assayed concurrently with each lot. Internal standard replaces USP/NF reference no longer available.

Physical form

Dialyzed, lyophilized powder, not less than300 units per mg (dry weight).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Aesthetic surgery journal, 33(8), 1167-1174 (2013-11-08)
Hyaluronidases, a family of enzymes that are able to degrade hyaluronic acid (HA), are employed in medicine to increase drug diffusion and reverse the effects of HA filler injections. Hyaluronidases are able to dissolve subcutaneous nodules or to correct excessive
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