MAB1281

Anti-Nuclei Antibody, clone 235-1

clone 235-1, Chemicon^®, from mouse

• Synonym(s): HuNu
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 235-1, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable
• input: sample type neural stem cell(s)
• isotype: IgG1
• shipped in: dry ice

Description

General description

Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models.

Specificity

Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). The antibody does not react with nuclei from mouse or rat.

The human nuclei monoclonal reacts specifically with human cells. It shows no reactivity against mouse, rat, chicken in immunohistorchemistry. Other mammalian or animal species have not been tested for reactivity.

Packaging

100 μL

Application

Detect Nuclei using this Anti-Nuclei Antibody, clone 235-1 validated for use in IH(P) & IP.

Immunohistochemistry/cytochemistry: 4% PFA fixed cryosections, or 5-10 minuted fixed cell cultures. Acetone fixation is also effective. Permeabilization with 0.1-0.4% triton X-100 is suggested in the blocking buffer. Dilution will vary slightly with lot; use at 1:10-1:500.

Formalin fixed, paraffin embedded tissues: 1:20 for 1hr at RT. Citrate buffer epitope retrieval method recommended.
http://www.ihcworld.com/_protocols/antibody_protocols/nuclei_chemicon.htm

Identifies an 82 and 70 kD protein by immunoprecipitation.

Optimal working dilutions and protocols must be determined by the end user.

Research Category
Stem Cell Research

Research Sub Category
Neural Stem Cells

Physical form

Format: Purified

Hybridoma supernatant. Liquid in PBS. Contains no preservative.

Protein A purified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
All human cell types

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable