Centricon-70 devices require a swinging bucket rotor with adapters for flat-bottom 250 ml tubes/bottles.
UFC710008
Centricon® Plus Centrifugal Filter
MWCO 100 kDa, sample volume 15-70 mL, regenerated cellulose membrane
Synonym(s):
Centrifuge concentrator
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About This Item
UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polypropylene cap, polypropylene cup (for concentrate/retentate), polypropylene cup (for filtrate collection), polypropylene cup (for sample filter), regenerated cellulose membrane
Pore size:
100 kDa MWCO
Working volume:
≤70 mL , 15—70 mL
Sterility:
non-sterile
Packaging:
pkg of 8 filter
Pricing and availability is not currently available.
Quality Level
material
polypropylene cap, polypropylene cup (for concentrate/retentate), polypropylene cup (for filtrate collection), polypropylene cup (for sample filter), regenerated cellulose membrane
sterility
non-sterile
product line
Amicon®
feature
holdup volume 75 μL
packaging
pkg of 8 filter
manufacturer/tradename
Amicon® Ultra, Centricon®
parameter
15-70 mL sample volume
technique(s)
protein extraction: suitable, protein purification: suitable
L
121 mm
diam.
6 cm
filtration area
19 cm2
working volume
≤70 mL , 15—70 mL
pore size
100 kDa MWCO
application(s)
sample preparation
shipped in
ambient
General description
Filter Type: Ultrafiltration
The Centricon Plus-70 centrifugal filter is a disposable, single-use device designed for the processing of aqueous biological solutions in volumes ranging from 15 to 70 mL.
The Centricon Plus-70 centrifugal filter is a disposable, single-use device designed for the processing of aqueous biological solutions in volumes ranging from 15 to 70 mL.
Application
CentriconPlus-70 Centrifugal Filter is suitable for the concentration and purification of biological samples.
Features and Benefits
- compatiblewith swinging-bucket centrifuges only
- can concentrate most 70 mL solutionsdown to 350 µL in 15-40 minutes
Physical form
Color Code: Clear
Other Notes
For research use only. Not for use in diagnostic procedures.
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
CENTRICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| parameter 15-70 mL sample volume | parameter 15-70 mL sample volume | parameter 15-70 mL sample volume | parameter 15-70 mL sample volume |
| manufacturer/tradename Amicon® Ultra, Centricon® | manufacturer/tradename Amicon® Ultra, Centricon® | manufacturer/tradename Amicon® Ultra, Centricon® | manufacturer/tradename Amicon® Ultra, Centricon® |
| material polypropylene cap, polypropylene cup (for filtrate collection), regenerated cellulose membrane, polypropylene cup (for concentrate/retentate), polypropylene cup (for sample filter) | material polypropylene cap, polypropylene cup (for concentrate/retentate), polypropylene cup (for filtrate collection), polypropylene cup (for sample filter), regenerated cellulose membrane | material polypropylene cap, polypropylene cup (for concentrate/retentate), polypropylene cup (for filtrate collection), polypropylene cup (for sample filter), regenerated cellulose membrane | material polypropylene cap, polypropylene cup (for concentrate/retentate), polypropylene cup (for filtrate collection), polypropylene cup (for sample filter), regenerated cellulose membrane |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| pore size 100 kDa MWCO | pore size 10 kDa MWCO | pore size 3 kDa MWCO | pore size 30 kDa MWCO |
| working volume ≤70 mL , 15—70 mL | working volume ≤70 mL , 15—70 mL | working volume ≤70 mL , 15—70 mL | working volume ≤70 mL , 15—70 mL |
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Storage Class
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Ultrafiltration-based MAb purification offers faster, higher recovery compared to hybridoma screening.
Articles
Auf dieser Seite werden die Grundsätze und Vorteile der Verwendung von Ultrafiltrationsmembranen bei der Vorbereitung von Proteinproben erörtert. Mögliche Anwendungen sind der Austausch von Lösungsmitteln, die Konzentration von Proteinproben oder die Probenaufreinigung.
Large-volume centrifugal filter devices are a convenient alternative to stirred cell ultrafiltration devices for purifying proteins from large volumes of solution.
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| UFC710008 | 08436037126130 |
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What are the adapter required for the swinging bucket centrifuge to use this? Can't find the recommended product anywhere.
1 answer-
Helpful?
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Was für ein Rotor wird für die Filter benötigt? What kind of rotor is required for the filter units?
1 answer-
This product is compatible with swinging-bucket capable of handling 250 mL bottles. Do not use a fixed angle rotor. Kindly review more technical specifications in the User Guide under "More Documents" or at this link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/866/336/pr03221w-ms.pdf
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What are the proposed adapters for centrifugation? We have a swing-bucket centrifuge in the lab, but we don´t have the proper bucket for it.
1 answer-
This device is suitable for swinging bucket rotors only. These centrifugal filters measure 121 mm (length) x 60 mm (diameter) and have a maximum g-force of 3500 x g. See the link below to review the recommended rotors and adaptor for several major brands of centrifuge:
https://www.emdmillipore.com/life-science-research/protein-sample-preparation/rotor-guides/Centricon-Plus-70/2B2b.qB.isYAAAFEcTQMfo3B,navHelpful?
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What is the maximum this device can be spun without damaging the membrane?
1 answer-
The maximum centrifugal force for concentrating is 3,500 × g. For more details please refer to the link below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/866/336/pr03221w-ms.pdf
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