Merck
All Photos(1)

11204530001

Roche

ABTS Buffer

solution, pkg of 125 mL

Synonym(s):
buffer

form

solution

Quality Level

packaging

pkg of 125 mL

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

General description

2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts as a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.[1]

Application

ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme.[2][3][4] Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.

Preparation Note

Working concentration: Flow cytometry: 0.5 μg/100 μl (106 cells); Immunohistocytochemistry: 50 μg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of ABTS buffer. This solution has a light-green color. In microtiter plates, the solution is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ABTS is a trademark of Roche

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service