A8456

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride

≥97.0% (HPLC)

• Synonym(s): AEBSF
• Empirical Formula (Hill Notation): C₈H₁₀FNO₂S · HCl
• CAS Number: 30827-99-7
• Molecular Weight: 239.69
• Beilstein: 7604627
• MDL number: MFCD00132962
• UNSPSC Code: 12352202
• PubChem Substance ID: 24278240
• NACRES: NA.77

Properties

• biological source: synthetic (organic)
• Quality Level: 300
• Assay: ≥97.0% (HPLC)
• form: powder
• mp: 183-191 °C
• solubility: H₂O: 50 mg/mL
• storage temp.: −20°C
• SMILES string: FS(C1=CC=C(CCN)C=C1)(=O)=O.[H]Cl
• InChI: 1S/C8H10FNO2S.ClH/c9-13(11,12)8-3-1-7(2-4-8)5-6-10;/h1-4H,5-6,10H2;1H
• InChI key: WRDABNWSWOHGMS-UHFFFAOYSA-N

Description

General description

stable for up to six months if stored refrigerated at a pH of less than 7. If a pH of greater than 7 is required, pH adjustment should be made just prior to use.

Application

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) has been used:

• as a protease inhibitor in plasma samples to prevent acylated ghrelin (AG) degradation
• as a snake venom serine proteinase (SVSP) inhibitor in SVSP inhibition or AEBSF assay to test the involvement of SVSPs on the fibrinogen-clotting activity
• as a component in NP-40 lysis buffer to resuspend cell pellets for preparing cell lysates

Recommended concentrations for use are 0.1-1 mM.

Biochem/physiol Actions

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) is an irreversible serine protease inhibitor. Inhibition constants are similar to those of phenylmethanesulfonyl fluoride (PMSF) and diisopropylfluorophosphate (DFP). AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein, and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water, and improved stability in aqueous solutions. AEBSF can be used in cell culture in concentrations of up to 0.25 mM.

AEBSF can inhibit acetylcholinesterase (AChE).

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H314
• Precautionary Statements: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1A
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable