Merck
All Photos(1)

B8806

Sigma-Aldrich

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester

1 mg/mL in DMSO

Synonym(s):
BCECF-AM solution, Spiro(isobenzofuran-1(3H),9′-(9H)xanthene)-2′,7′-dipropanoic acid
EC Number:
NACRES:
NA.47

description

Mixture of six isomeric compounds

Quality Level

form

liquid

concentration

1 mg/mL in DMSO

color

colorless to yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CC(=O)OCOC(=O)CCc1cc2c(Oc3cc(OCOC(C)=O)c(CCC(=O)OCOC(C)=O)cc3C24OC(=O)c5cc(ccc45)C(=O)OCOC(C)=O)cc1OCOC(C)=O

Application

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester has been used for the estimation of vacuolar pH and intracellular pH.

Biochem/physiol Actions

An intracellular fluorescent pH indicator.
Hydrolyzed to BCECF by cytosolic esterases.

Physical form

Solution in 0.1% BCECF AM Ester and 99.9% DMSO

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

185.0 °F

Flash Point(C)

85 °C

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service