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C1359

Sigma-Aldrich

Calcein AM solution

≥90% purity (HPLC), solution

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100 μL
CHF 550.00

CHF 550.00


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100 μL
CHF 550.00

About This Item

Empirical Formula (Hill Notation):
C46H46N2O23
CAS Number:
Molecular Weight:
994.86
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

CHF 550.00


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Product Name

Calcein AM solution, 4 mM in DMSO, ≥90% (HPLC), solution

Quality Level

Assay

≥90% (HPLC)

form

solution

concentration

4 mM in DMSO

technique(s)

titration: suitable

color

colorless
white to off-white

λmax

491-497 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45

InChI

1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3

InChI key

XKFSBWQWNMZWFA-UHFFFAOYSA-N

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This Item
1778356496206700
Calcein-AM BioReagent, suitable for fluorescence, ≥90% (HPLC)

17783

Calcein-AM

Calcein-AM Small Package (20 X 50 μg ), ≥90.0% (HPLC)

56496

Calcein-AM

technique(s)

titration: suitable

technique(s)

-

technique(s)

-

technique(s)

-

color

colorless, white to off-white

color

-

color

-

color

off-white

concentration

4 mM in DMSO

concentration

-

concentration

-

concentration

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

application(s)

-

form

solution

form

-

form

-

form

solid

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Application

In cells, calcein AM solution has been used in calcein-AM dye transfer assay.[1] It has been used for the staining of cells to study quantification of cell-migration.[2] It has been used to study cytotoxic effects of drugs in cells.[3]

Biochem/physiol Actions

Calcein AM is a membrane permeable nonfluorescent molecule. It is converted to fluorescent calcein by intracellular esterases. Calcein has limited membrane permeability. Calcein AM is also a substrate for p-glycoprotein (Pgp). Presence of Pgp results in efflux of calcein AM and thereby decreases cellular fluorescence. On the other hand, presence of Pgp inhibitor increases cellular entry of calcein AM and thereby increases cellular fluorescence. Calcein AM plays an important role in early drug discovery.[4]
Fluorescent cell permeable derivative of calcein.

Physical form

Solution in 0.4% Calcein AM and 99.6% DMSO.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

185.0 °F - closed cup

Flash Point(C)

85 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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