Merck
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D7008

Sigma-Aldrich

Dihydroethidium

≥95%

Synonym(s):
2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine
Empirical Formula (Hill Notation):
C21H21N3
CAS Number:
Molecular Weight:
315.41
Beilstein:
5107482
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

Assay

≥95%

form

powder

solubility

chloroform: 20 mg/mL

fluorescence

λex 370 nm; λem 420 nm (for cytoplasm of living cells)
λex 535 nm; λem 610 nm (for chromatin of living cells)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14

InChI

1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3

InChI key

XYJODUBPWNZLML-UHFFFAOYSA-N

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Dihydroethidium ≥95%

Sigma-Aldrich

D7008

Dihydroethidium

Dihydrorhodamine 123 ≥95%

Sigma-Aldrich

D1054

Dihydrorhodamine 123

Dihydroethidium BioReagent, suitable for fluorescence, ≥95% (HPCE)

Sigma-Aldrich

37291

Dihydroethidium

assay

≥95%

assay

≥95%

assay

99%

assay

≥95% (HPCE)

form

powder

form

powder

form

-

form

solid

solubility

chloroform: 20 mg/mL

solubility

DMSO: >5 mg/mL

solubility

methanol: 25 mg/mL, clear, colorless to yellow

solubility

acetonitrile: soluble, methanol: soluble

fluorescence

λex 370 nm; λem 420 nm (for cytoplasm of living cells), λex 535 nm; λem 610 nm (for chromatin of living cells)

fluorescence

-

fluorescence

-

fluorescence

λex 358 nm; λem 461 nm, λex 392 nm; λem 410 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

room temp

storage temp.

−20°C

Application

Redox indicator. Blue fluorescence until oxidized to ethidium.
Dihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC). It has been used to check the in situ generation of ROS (reactive oxygen species).

Packaging

Packaged under Argon.

Linkage

B-ring reduced analog of ethidium

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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