Heparin sodium salt from porcine intestinal mucosa is soluble in deionized water at a concentrationi of 50 mg/mL.
Solutions at 0.5 mg per mL were tested in cell culture. Start at this concentration, and titer from there to determine the optimal concentration for usage.
Solutions can be stored at 2-8C for up to 2 years, if sterile filtered through a 0.22 micron filter. Benzyl alcohol (0.06-1%) can be added to solutions to prevent microbial growth.
The potency, in units/mg, is lot-specific; the value can be found on the certificate of analysis. It is typically over 140 units per mg. The number of milligrams can be calculated by dividing the number of units by the potency in units per mg.
Heparin is a mixture of polyanion chains having molecular weights ranging from 6,000 to 30,000 Daltons, with most chains in the range of 17,000 to 19,000 Daltons.
We suggest using 20 to 50 units per mL of blood.
Although solutions may be autoclaved at 121 °C for 5 to 10 minutes, we generally recommend sterile filtration. This is because prolonged autoclaving may have an effect on its activity.
Heparin has been used at concentrations ranging from 100 to 500 μg/mL. This has been reported in the J. Biol. Chem., 248, 2095 (1973).
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.