All Photos(2)



Propidium iodide solution

solution (1.0 mg/ml in water)

3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide, 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium, diiodide
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
MDL number:
PubChem Substance ID:



Quality Level


solution (1.0 mg/ml in water)


water: 1 mg/mL


5400-6400 at 491-495 nm in phosphate buffer


diagnostic assay manufacturing

storage temp.


SMILES string




InChI key


Looking for similar products? Visit Product Comparison Guide

General description

Propidium iodide solution is used to assess neuronal death.


Propidium iodide solution has been used:
  • in staining DNA
  • in the viability staining method to study islet viability
  • to visualize F-actins and nuclei in mesenchymal stem cell sheets

Membrane impermeant, phenanthridinium intercalator dye excited by mercury or xenon-arc lamps or argon-ion laser. Suitable for use in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry. Used as a nuclear counterstain.


10 mL in glass bottle

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Does Propidium Iodide, Product P4864, stain viable or non-viable cells?

    Propidium Iodide does not generally enter living cells,  However it may be taken up by axonal terminals and retrogradely transported to neuronal cell bodies. Reference: Aschoff, A. and H. Hollander, Fluorescent compounds as retrograde tracers compared with horseradish peroxidase (HRP). I. A parametric study in the central visual system of the albino rat. J. Neurosci. Methods, 6(3), 179-197 (1982).

  4. What are the excitation and emission maxima for Propidium Iodide, Product P4864?

    Excitation maximum is at 493 nm in water.  Emission maximum is at 636 nm in water

  5. How is Propidium Iodide, Product P4864, used for Flow Cytometry?

    Immediately before flow cytometric analysis, prepare a 0.5 mg/mL solution of propidium iodide by combining 50μL of 2X PBS with 50 μL of P4864. Add this solution to 1 mL of a cell suspension containing 10,000,000 cells. (Note: Cells exhibiting fluorescence above 630 nm should be excluded from further analysis.)

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Variation in human islet viability based on different membrane integrity stains
Barnett MJ, et al.
Cell Transplantation, 13(5), 481-488 (2004)
Stroke E-Book: Pathophysiology Diagnosis and Management, 79-79 (2011)
Paternal cyclophosphamide exposure induces the formation of functional micronuclei during the first zygotic division
Grenier L, et al.
PLoS ONE, 6(11), e27600-e27600 (2011)
Direct intramyocardial injection of mesenchymal stem cell sheet fragments improves cardiac functions after infarction
Wang CC, et al.
Cardiovascular Research, 77(3), 515-524 (2007)
Chung-Chi Wang et al.
Cardiovascular research, 77(3), 515-524 (2007-11-17)
Cell transplantation is a promising approach for patients with myocardial infarction. However, following injection, retention of the transplanted cells in the injected area remains a central issue, which can be deleterious to cell transplantation therapy. We hypothesized that the use


Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Related Content

DNA Detection

Nancy-520 for DNA Detection and Quantitation

Preparation & Staining of 3D Printed Tissues & Scaffolds

Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service