average mol wt 30,000-70,000
4-6 cP, 4 % in H2O(20 °C)(lit.)
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Polyvinyl alcohol is normally prepared by hydrolysis of the acetate, CH3(C=O)O, groups of polyvinylacetate, to leave behind the polyvinyl backbone with the OH groups. The expected degree of acetate hydrolysis is 87-90%. This means that 87-90% of the side groups are OH, while the remaining 10-13% side groups are CH3(C=O)O.
MALLS stands for Multi-Angle Laser Light Scattering. Sometimes now called Multiangle Light Scattering (MALS), this technique allows for the determination of a MW range and particle size range of a given species in solution.
In general, PVA solutions are very viscous, and sterile-filtering would probably be quite difficult. It might be better to coat the surface of interest and then irradiate the coated surface under UV light, such as in a cell culture hood.
We have not specifically tested P8136 for use with cultured cells. This is also not a sterile product. However, several publications claim use of P8136 in culture work.
Product P8136, poly(vinyl alcohol), should be solubilized in cold water with adequate agitation to ensure complete wetting of the particles. (That is, keep the particles suspended with gentle stirring.) The temperature should then be increased to 85°C for 30 minutes to ensure complete dissolution. Product P8136 has been found to be soluble in water at a concentration of 50 mg/mL, yielding a clear to slightly hazy, colorless to faint yellow solution.
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