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R8881

Sigma-Aldrich

Rhodamine B isothiocyanate–Dextran

average mol wt ~10,000

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Synonym(s):
RITC-Dextran
MDL number:
NACRES:
NA.47

form

powder

Quality Level

mol wt

average mol wt ~10,000

extent of labeling

0.002-0.01 mol RITC per mol glucose

color

light pink to red
very dark purple

solubility

water: 10 mg/mL, clear, pink

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

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This Item
R9379R6626283924
vibrant-m

R6626

Rhodamine B

vibrant-m

283924

Rhodamine B isothiocyanate

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

-

form

powder

form

powder

solubility

water: 10 mg/mL, clear, pink

solubility

-

solubility

H2O: 1 mg/mL

solubility

methanol: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

General description

Rhodamine B is a thiol-reactive and photostable fluorescent dye. Rhodamine B isothiocyanate is an aminoxanthene that possesses carboxylic acid and isothiocyanate substituents on the pendant phenyl ring.

Application

Rhodamine B isothiocyanate (NE)-labeled dextran is often used as a fluorescent probe for studies of endocytotic activity and to detect the migrated of marked cells. It is also suitable for perfusion studies in animals. Rhodamine B isothiocyanate–Dextran has been used for in vivo zebrafish blood-brain barrier (BBB) dye diffusion assay.
Dextran labeled with rhodamine B isothiocyanate for possible use in perfusion studies in animals.

Biochem/physiol Actions

Rhodamine B stains the tissue in red-violet color and emits a bright red color when fluoresced. It is highly preferred for marking insects.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Xiaotong Wu et al.
Journal of molecular cell biology, 10(6), 479-493 (2018-07-31)
Oocytes, the irreplaceable gametes for generating a new organism, are matured in the ovary of living female animals. It is unknown whether any genetic manipulations can be applied to immature oocytes inside the living ovaries. As a proof-of-concept, we here
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Zhuangbin Liao
Neuroscience (2016)
Jeremy Gingrich et al.
Chemosphere, 263, 128304-128304 (2020-11-07)
Gap junction intercellular communication (GJIC) is necessary for ovarian function, and it is temporospatially regulated during follicular development and ovulation. At outermost layer of the antral follicle, theca cells provide structural, steroidogenic, and vascular support. Inter- and extra-thecal GJIC is
Ruitao Su et al.
Science advances, 6(41) (2020-10-11)
Microfluidic devices fabricated via soft lithography have demonstrated compelling applications such as lab-on-a-chip diagnostics, DNA microarrays, and cell-based assays. These technologies could be further developed by directly integrating microfluidics with electronic sensors and curvilinear substrates as well as improved automation
Jiawei Sun et al.
Development (Cambridge, England), 145(19) (2018-08-24)
Maternal mRNAs and proteins dictate early embryonic development before zygotic genome activation. In the absence of transcription, elaborate control of maternal mRNA translation is of particular importance for oocyte maturation and early embryogenesis. By analyzing zebrafish ybx1 mutants with a

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