Merck
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SE100

Sigma-Aldrich

Silver Enhancer Kit

All Photos(1)
NACRES:
NA.47

form

liquid

Quality Level

200

usage

sufficient for ≥100 slides staining and fixing

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

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application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

Quality Level

200

Quality Level

500

Quality Level

200

Quality Level

400

form

liquid

form

solution

form

-

form

-

usage

sufficient for ≥100 slides staining and fixing

usage

-

usage

-

usage

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

15-25°C

Application

Silver Enhancer Kit has been used to determine the presence of gold nanorods in various tissues and to prepare silver enhanced gold colloids for electron microscopy.

Features and Benefits

Colloidal gold labels are normally visible only at the electron microscope level. Silver Enhancer enlarges the gold colloid label by precipitation of metallic silver to give a high contrast signal visible under a light microscope. A darkroom is not necessary for this procedure, as the silver enhancer working solution is light-stable for 20-30 minutes under ordinary laboratory lighting.

Packaging

The Silver Enhancer kit consists of Solution A (silver salt), Solution B (initiator), Sodium Thiosulfate (fixer), and a Technical Bulletin describing their use.

Principle

Solution A and Solution B are mixed 1:1 immediately before use and applied to the slide holding the gold-labeled section. After 5-10 minutes, the slide is rinsed with distilled water, fixed for 2-3 minutes in a sodium thiosulfate solution, and rinsed again. The section is now ready for counterstaining and mounting as required.

Kit Components Only

Product No.
Description
  • Sodium thiosulfate pentahydrate, reagent grade, ≥99.5% 500 g

Kit Components Also Available Separately

Product No.
Description
SDS
  • S5020Silver enhancer solution A 100 mLSDS
  • S5145Silver enhancer solution B 100 mLSDS

Pictograms

Environment
GHS09

Signal Word

Warning

Hazard Statements

H410

Precautionary Statements

P273 - P391 - P501

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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