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295876

Sigma-Aldrich

Formamide

spectrophotometric grade, ≥99%

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Synonym(s):
Amide C1, Formic amide
Linear Formula:
HCONH2
CAS Number:
Molecular Weight:
45.04
Beilstein:
505995
EC Number:
MDL number:
PubChem Substance ID:

grade

spectrophotometric grade

Quality Level

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

Assay

≥99%

form

liquid

autoignition temp.

932 °F

expl. lim.

2.7-19 %

technique(s)

UV/Vis spectroscopy: suitable
hybridization: suitable

impurities

<0.030% water

refractive index

n20/D 1.447 (lit.)

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

density

1.134 g/mL at 25 °C (lit.)

λ

H2O reference

UV absorption

λ: 275 nm Amax: 1.00
λ: 280 nm Amax: 0.20
λ: 300 nm Amax: 0.05
λ: 360 nm Amax: 0.02
λ: 400 nm Amax: 0.01

application(s)

food and beverages

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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47671F9037F7503
Formamide spectrophotometric grade, &#8805;99%

Sigma-Aldrich

295876

Formamide

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Formamide BioUltra, for molecular biology, &#8805;99.5% (T)

Sigma-Aldrich

47671

Formamide

Premium Grade
Formamide &#8805;99.5% (GC), BioReagent, for molecular biology

Sigma-Aldrich

F9037

Formamide

-
Formamide ReagentPlus&#174;, &#8805;99.0% (GC)

Sigma-Aldrich

F7503

Formamide

-
vapor density

1.55 (vs air)

vapor density

1.55 (vs air)

vapor density

1.55 (vs air)

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

assay

≥99%

assay

≥99.5% (T)

assay

≥99.5% (GC)

assay

≥99.0% (GC)

form

liquid

form

liquid

form

liquid

form

liquid

autoignition temp.

932 °F

autoignition temp.

932 °F

autoignition temp.

932 °F

autoignition temp.

932 °F

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Target Organs

Blood

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

305.6 °F

Flash Point(C)

152 °C


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Alzheimer's disease (AD) is a premature ageing syndrome characterized by cognitive impairment arising from neuropathological changes occurring within specific areas of the brain. We report a 1.5-fold increase in trisomy 21 (P < 0.001) and a 1.2-fold increase in trisomy
The formamide method for the extraction of polysaccharides from haemolytic streptococci.
Fuller AT.
British Journal of Experimental Pathology, 32(1), 130-130 (1960)
Solubilization in formamide protects RNA from degradation.
Chomczynski P.
Nucleic Acids Research, 20(14), 3791-3791 (1992)
Microwave spectrum and molecular structure of formamide.
Costain CC and Dowling JM.
J. Chem. Phys. , 32(1), 158-165 (1960)
J Casey et al.
Nucleic acids research, 4(5), 1539-1552 (1977-01-01)
The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is

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