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34877

Sigma-Aldrich

Water

HPLC Plus

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Linear Formula:
H2O
CAS Number:
7732-18-5
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
231-791-2
MDL number:
MFCD00011332
PubChem Substance ID:
329755083
NACRES:
NA.21

grade

HPLC Plus

Quality Level

100

vapor density

<1 (vs air)

vapor pressure

3 mmHg

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable

impurities

≤0.0003% non-volatile matter
≤1 ppb fluorescence (quinine) at 254 nm
≤1 ppb fluorescence (quinine) at 365 nm

refractive index

n20/D 1.34 (lit.)

pH

6.0-8.0 (25 °C)

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

anion traces

chloride (Cl-): ≤0.1 mg/kg
fluoride (F-): ≤0.1 mg/kg
nitrate (NO3-): ≤0.1 mg/kg
sulfate (SO42-): ≤0.1 mg/kg

HPLC-gradient

≤1 mAU at 254 nm
≤5 mAU at 210 nm

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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This Item
W175434877-M95283
Water HPLC Plus

Sigma-Aldrich

34877

Water

Water PCR Reagent

Sigma-Aldrich

W1754

Water

Water HPLC Plus

Sigma-Aldrich

34877-M

Water

Water for HPCE, for luminescence, suitable for UV/Vis spectroscopy

Supelco

95283

Water

vapor density

<1 (vs air)

vapor density

<1 (vs air)

vapor density

-

vapor density

-

vapor pressure

3 mmHg

vapor pressure

3 mmHg

vapor pressure

-

vapor pressure

-

form

liquid

form

liquid

form

liquid

form

-

technique(s)

HPLC: suitable

technique(s)

PCR: suitable

technique(s)

-

technique(s)

UV/Vis spectroscopy: suitable

impurities

≤0.0003% non-volatile matter, ≤1 ppb fluorescence (quinine) at 254 nm, ≤1 ppb fluorescence (quinine) at 365 nm

impurities

-

impurities

≤0.0003% non-volatile matter, ≤1 ppb fluorescence (quinine) at 254 nm, ≤1 ppb fluorescence (quinine) at 365 nm

impurities

-

General description

HPLC Plus grade water is useful for the UHPLC (Ultra-High Pressure Liquid Chromatography) gradient elution and HPLC studies.

Application

Water has been used in the following studies:
  • To prepare the diluted hydrochloric acid solutions required for the preparation of standard solutions of amino acid.
  • In the sample extraction step during the gas chromatography mass spectrometric (GC-MS) analysis of free (FAA) and combined amino acids (CAA) in aerosol samples.
  • Preparation of tryptic digests from the standard protein samples and milk digests from milk samples.
  • Preparation of C60-aminopropylsilica.

Preparation Note

Product filtered through a 0.2 μm filter

Other Notes

Important notice
  • The article number 34877-4X2.5L will be discontinued. Please order the single bottle 34877-2.5L which is physically identical with the same exact specifications.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available

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Manolis Mandalakis et al.
Journal of chromatography. A, 1217(1), 143-150 (2009-12-08)
The analysis of amino acids by gas chromatography mass spectrometry (GC-MS) after their derivatization with N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide was investigated as an alternative approach for the determination of free (FAA) and combined amino acids (CAA) in aerosols. This technique showed excellent linearity
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Analytica chimica acta, 761, 92-101 (2013-01-15)
Phosphorylation of proteins is an important cellular regulatory process. The analysis of protein phosphorylation is challenging due to the high dynamic range and low abundance natures of phosphorylated species. Mass spectrometry (MS) of phosphopeptides obtained from tryptic protein digests is
Mary A Rodgers et al.
The Journal of experimental medicine, 211(7), 1333-1347 (2014-06-25)
Linear ubiquitination is a newly discovered posttranslational modification that is currently restricted to a small number of known protein substrates. The linear ubiquitination assembly complex (LUBAC), consisting of HOIL-1L, HOIP, and Sharpin, has been reported to activate NF-κB-mediated transcription in
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