21888

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

• Synonym(s): CFSE
• Empirical Formula (Hill Notation): C₂₉H₁₉NO₁₁
• CAS Number: 150347-59-4
• Molecular Weight: 557.46
• MDL number: MFCD00037466
• UNSPSC Code: 12352108
• PubChem Substance ID: 57647951
• NACRES: NA.32

Properties

• product line: BioReagent
• Assay: ≥90% (HPLC)
• form: solid
• mp: 152-154 °C (lit.)
• solubility: DMF: soluble; DMSO: soluble
• fluorescence: λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0 (esterase)
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7
• InChI: 1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3
• InChI key: LZFDYQHOQDFMOA-UHFFFAOYSA-N

Description

General description

5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.[1]

Application

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry.[2][3] Forms green fluorescent conjugates on deacetylation.

5[4]-Carboxyfluorescein diacetate N-succinimidyl ester has been used:

• to label cells for flow cytometry[5][6]
• in lymphocyte proliferation assay[7][8]
• to determine the intracellular or intra-organellar pH in chloroplasts[4]
• to label Leishmania amazonensis and Leishmania major[9]

Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.

Biochem/physiol Actions

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.[10]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves