Merck
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37291

Sigma-Aldrich

Dihydroethidium

BioReagent, suitable for fluorescence, ≥95% (HPCE)

Synonym(s):
Hydroethidine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine
Empirical Formula (Hill Notation):
C21H21N3
CAS Number:
Molecular Weight:
315.41
Beilstein:
5107482
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Quality Level

product line

BioReagent

assay

≥95% (HPCE)

form

solid

solubility

acetonitrile: soluble
methanol: soluble

fluorescence

λex 358 nm; λem 461 nm
λex 392 nm; λem 410 nm in methanol

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14

InChI

1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3

InChI key

XYJODUBPWNZLML-UHFFFAOYSA-N

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Application

Dihydroethidium (dHEt), a fluorescene probe, is used to detect the presence of reactive oxygen species (ROS) such as superoxides.
Redox indicator. Blue fluorescence until oxidized to ethidium.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Linkage

B-ring reduced analog of ethidium

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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