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41176

Sigma-Aldrich

Anti-Rabbit IgG - Atto 633 antibody produced in goat

1 mg/mL IgG

Synonym(s):
Atto 633 [goat-Anti-rabbit IgG]
NACRES:
NA.46

Quality Level

biological source

goat

conjugate

Atto 633 conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

contains

50% glycerol as stabilizer

species reactivity

rabbit

concentration

1 mg/mL IgG

technique(s)

immunohistochemistry: 1:200

fluorescence

λex 636 nm; λem 650 nm in PBS

storage temp.

−20°C

Related Categories

General description

Anti-rabbit IgG (whole molecule) (Sigma, R4880) is developed in goat using purified rabbit IgG as the immunogen. Affinity isolated antigen specific antibody is purified from goat anti-rabbit IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to rabbit IgG. Goat anti-rabbit is conjugated to Atto 633 NHS (Abs.max. 629 nm; Em.max. 657 nm), (Cat. No. 01464), then further purified via gel permeation chromatography and dialysis to remove unbound Atto-Dye.

Immunogen

purified rabbit IgG

Application

Goat anti-rabbit IgG can be used for immunohistochemical applications at a dilution of 1:200.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Maëva Clerte et al.
Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography, 26(12), 1465-1473 (2013-09-03)
When activated by the sympathetic nervous system, brown adipose tissue (BAT) increases energy expenditure to produce heat. Augmenting BAT mass or increasing BAT activation could potentially be used to decrease obesity. Noninvasive methods to detect and monitor BAT mass are
Huildore Bommanna Ranjitha et al.
Journal of cell science, 134(5) (2020-06-03)
Foot-and-mouth disease virus (FMDV) is a picornavirus that causes contagious acute infection in cloven-hoofed animals. FMDV replication-associated viral protein expression induces endoplasmic reticulum (ER) stress and the unfolded protein response (UPR), in turn inducing autophagy to restore cellular homeostasis. We
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S N Suresh et al.
Frontiers in molecular neuroscience, 11, 109-109 (2018-04-25)
Mechanistic insights into aggrephagy, a selective basal autophagy process to clear misfolded protein aggregates, are lacking. Here, we report and describe the role of Estrogen Related Receptor α (ERRα, HUGO Gene Nomenclature ESRRA), new molecular player of aggrephagy, in keeping
Somya Vats et al.
Molecular biology of the cell, 30(17), 2283-2295 (2019-06-13)
Autophagy is an evolutionarily conserved intracellular lysosomal degradation pathway. It is a multistep process involving de novo formation of double membrane autophagosomes that capture cytosolic constituents (cargo) and eventually fuse with lysosomes wherein the cargo gets degraded and resulting simpler

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