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71376

Sigma-Aldrich

Sodium chloride

BioUltra, for molecular biology, ≥99.5% (AT)

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Synonym(s):
Salt, Halite, NaCl
Linear Formula:
NaCl
CAS Number:
Molecular Weight:
58.44
Beilstein:
3534976
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

vapor pressure

1.33 (1.00 mmHg) at 865 °C hPa

product line

BioUltra

Assay

≥99.5% (AT)

form

solid

availability

not available in (Sales restrictions may apply)

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.001% total nitrogen (N)

pH

5.0-8.0 (25 °C, 1 M in H2O)

mp

801 °C (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

density

2.16 g/cm3 at 25 °C (77 °F)

anion traces

bromide (Br-): ≤50 mg/kg
hexacyanoferrate(II) ([Fe(CN)6]4-): ≤1 mg/kg
iodide (I-): ≤10 mg/kg
phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤200 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤1 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string

[Na+].[Cl-]

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.01
λ: 280 nm Amax: ≤0.01

suitability

suitable for molecular biology

InChI

1S/ClH.Na/h1H;/q;+1/p-1

InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

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This Item
S301471383S5150
Sodium chloride BioUltra, for molecular biology, ≥99.5% (AT)

Sigma-Aldrich

71376

Sodium chloride

Premium Grade
Sodium chloride for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

Sigma-Aldrich

S3014

Sodium chloride

-
Sodium chloride tested according to Ph. Eur.

Sigma-Aldrich

71383

Sodium chloride

-
Sodium chloride solution 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture

Sigma-Aldrich

S5150

Sodium chloride solution

Essential+ Grade
vapor pressure

1.33 (1.00 mmHg) at 865 °C hPa

vapor pressure

-

vapor pressure

1.33 (1.00 mmHg) at 865 °C hPa

vapor pressure

-

assay

≥99.5% (AT)

assay

≥99% (titration)

assay

99.7% (dried substance)

assay

-

form

solid

form

crystalline

form

solid

form

liquid

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.001% total nitrogen (N)

impurities

DNAse, none detected, Endonuclease, none detected, NICKase, none detected, RNAse, none detected, protease, none detected

impurities

-

impurities

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, Protease, none detected, RNAse, none detected, <1.0 EU/mL Endotoxin level, <16 cfu/mL Bioburden

pH

5.0-8.0 (25 °C, 1 M in H2O)

pH

7

pH

7

pH

6.0-8.0

General description

Sodium chloride, an essential nutrient, plays a critical role in biochemistry and molecular biology, making significant contributions to various biochemical applications. Within biological systems, it serves as a fundamental electrolyte, regulating osmotic pressure, pH balance, and electrical conductivity, all of which are essential for proper cellular function and vital physiological processes. Furthermore, sodium chloride plays a pivotal part in maintaining protein structure and promoting enzymatic activity, essential for stabilizing protein conformations and catalyzing biochemical reactions. Its role extends to the regulation of cellular homeostasis and the facilitation of ion and nutrient transport across cell membranes.It is an integral component of buffers like PBS and SSC, where it aids in various biochemical procedures, including the creation of density gradients and the fine-tuning of biological functions. Notably, sodium chloride is used to increase the ionic strength in buffers and culture media, impacting the ionic environment crucial for specific experiments and cell culture. Moreover, it is applied in DNA precipitation from samples containing SDS and the removal of small nucleic acid fragments during plasmid DNA preparations. Its utility extends to cell culture applications, where it plays a role in bacterial, yeast, and cell culture growth media.In molecular biology and cell culture, sodium chloride is an indispensable salt that finds its place in various aspects of research. It is a common constituent in cell culture media, lysis buffers, and protein storage solutions, contributing to the maintenance of optimal conditions for cell growth and experimentation. The sodium chloride used in these applications is of high purity, rigorously tested for impurities such as DNases, RNases, phosphatases, proteases, and trace metals, making it the ideal choice for molecular biology and biochemical research

Application

Sodium chloride has been used:

  • as a component of buffer solution to prepare the pH solutions for open-circuit potential and cyclic voltammetry
  • in the preparation of Bold’s basal medium (BBM)
  • as a component of transcription and annealing buffer
  • to maintain osmotic balance and ensure the purity and stability of the isolated extracellular vesicles (EVs)
  • as a buffer for the lamination of lateral flow strips in the fabrication of lateral flow devices

Biochem/physiol Actions

Sodium chloride helps to stimulate the stable induction of T-helper cell 17 (TH17) cells.

Features and Benefits

  • Suitable for molecular biology and biochecmial research
  • Free from DNase, RNase, NICKase and protease
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
  • Tested for the presence of Anionic and Cationic traces

Other Notes

This product is intended for research purposes only, and it is not meant for human consumption.
Component of lysis buffer and extraction buffer in the large-scale phenol extraction of RNA; Simple salting out procedure for extracting DNA from human nucleated cells
Sales restrictions may apply

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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A simple salting out procedure for extracting DNA from human nucleated cells.
S A Miller et al.
Nucleic acids research, 16(3), 1215-1215 (1988-02-11)
Sodium chloride drives autoimmune disease by the induction of pathogenic T H 17 cells
Kleinewietfeld M, et al.
Nature, 496(7446), 518-518 (2013)
D.M. Wallace
Methods in Enzymology, 152, 39-39 (1987)
A solid-state thin-film Ag/AgCl reference electrode coated with graphene oxide and its use in a pH sensor
Kim T, et al.
Sensors, 15(3), 6469-6482 (2015)
Quantitative detection of C-reactive protein in human saliva using an electrochemical lateral flow device
Petruzzi et al.
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