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Sigma-Aldrich

Osmium tetroxide solution

suitable for electron microscopy, 2% in H2O

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Synonym(s):
Osmium(IV) oxide
Linear Formula:
OsO4
CAS Number:
Molecular Weight:
254.23
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

solution

Quality Level

reaction suitability

reagent type: oxidant

concentration

2% in H2O

technique(s)

electron microscopy: suitable

color

colorless to yellow

density

1.0 g/mL at 20 °C
1.0 g/mL at 20 °C

application(s)

hematology
histology

storage temp.

2-8°C

SMILES string

O=[Os](=O)(=O)=O

InChI

1S/4O.Os

InChI key

VUVGYHUDAICLFK-UHFFFAOYSA-N

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This Item
O5500201030G7776
Osmium tetroxide Sealed ampule.

Sigma-Aldrich

O5500

Osmium tetroxide

Osmium tetroxide ReagentPlus®, 99.8%

Sigma-Aldrich

201030

Osmium tetroxide

density

1.0 g/mL at 20 °C

density

-

density

-

density

-

reaction suitability

reagent type: oxidant

reaction suitability

reagent type: oxidant

reaction suitability

reagent type: oxidant

reaction suitability

-

concentration

2% in H2O

concentration

-

concentration

-

concentration

70% in H2O

technique(s)

electron microscopy: suitable

technique(s)

-

technique(s)

-

technique(s)

electron microscopy: suitable (fixative)

color

colorless to yellow

color

-

color

-

color

colorless

General description

Osmium tetroxide (OsO4) also referred to as osmium, is a heavy tetrahedral, symmetrical molecule. It is both a dye and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium tetroxide to ensure the preservation of the lipids.

Application

Osmium tetroxide solution has been used as a fixative in electron microscopy.

Biochem/physiol Actions

Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.

Pictograms

CorrosionSkull and crossbones

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

6.1B - Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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