Merck
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79390

Sigma-Aldrich

Phosphatase, Alkaline from calf intestinal mucosa

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

Synonym(s):
Alkaline Phosphatase from calf intestinal mucosa
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
eCl@ss:
42010105
NACRES:
NA.54

biological source

bovine (calf) intestinal mucosa

form

solution (clear, colorless)

specific activity

~2500 U/mg protein (~10 mg/ml)

mol wt

Mr ~140000

technique(s)

enzyme immunoassay: suitable

impurities

0.1 mM ZnCl2
1 mM MgCl2
3 M NaCl
30 mM triethanolamine

pH

~7.6

storage temp.

2-8°C

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Application

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C

Reconstitution

The solution can be used without dialysis (for enzyme immuno-assay).

Other Notes

Sales restrictions may apply
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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